Wong C K, Leung K N, Fung K P, Choy Y M
Department of Biochemistry, Chinese University of Hong Kong, Shatin, 12 Territories.
Immunopharmacology. 1994 Jul-Aug;28(1):47-54. doi: 10.1016/0162-3109(94)90038-8.
We have previously shown that a mitogenic fraction (PH-I) separated from Pseudostellaria heterophylla exhibits both immunomodulatory and anti-tumor activities. In the present study, PH-I was further purified by gel filtration chromatography and the resulting three fractions (PH-I A, PH-I B and PH-I C) were assessed for their anti-tumor activity in vivo. It was found that fraction PH-I C from P. heterophylla could markedly suppress the growth of EAT cells in vivo. Mechanistic studies have shown that i.p. injection of PH-I C into mice could enhance the phagocytic activity of thioglycollate-elicited peritoneal macrophages. Moreover, PH-I C showed a potent activating effect on the cytotoxic activity of natural killer (NK) cells and alloreactive cytotoxic T cells (Tc) as well as increased the MurIL-2-induced lymphokine activated killer cell (LAK) activity in vitro. In addition, PH-I C could increase the number of tumor infiltrating lymphocytes (TILs) in the tumor site of WEHI-164-bearing mice. Finally, i.v. injection of PH-I C significantly elevated the levels of IFN-gamma and IL-4 in sera of EAT-bearing mice.
我们之前已经表明，从太子参中分离出的一种促有丝分裂组分（PH-I）具有免疫调节和抗肿瘤活性。在本研究中，通过凝胶过滤色谱法对PH-I进行了进一步纯化，并对所得的三个组分（PH-I A、PH-I B和PH-I C）进行了体内抗肿瘤活性评估。结果发现，太子参中的组分PH-I C能够在体内显著抑制艾氏腹水瘤（EAT）细胞的生长。机制研究表明，向小鼠腹腔注射PH-I C可增强巯基乙酸诱导的腹腔巨噬细胞的吞噬活性。此外，PH-I C对自然杀伤（NK）细胞和同种异体反应性细胞毒性T细胞（Tc）的细胞毒性活性具有强大的激活作用，并且在体外增加了MurIL-2诱导的淋巴因子激活的杀伤细胞（LAK）活性。此外，PH-I C可增加携带WEHI-164肿瘤小鼠肿瘤部位的肿瘤浸润淋巴细胞（TILs）数量。最后，静脉注射PH-I C显著提高了携带EAT小鼠血清中IFN-γ和IL-4的水平。
