InvB是肠炎沙门氏菌效应蛋白SopE的一种III型分泌相关伴侣蛋白。
InvB is a type III secretion-associated chaperone for the Salmonella enterica effector protein SopE.
作者信息
Lee Sang Ho, Galán Jorge E
机构信息
Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut 06536, USA.
出版信息
J Bacteriol. 2003 Dec;185(24):7279-84. doi: 10.1128/JB.185.24.7279-7284.2003.
Abstract
SopE is a bacteriophage-encoded effector protein of Salmonella enterica serovar Typhimurium that is translocated into the cytosol of eukaryotic cells by a type III secretion system (TTSS) (W.-D. Hardt, H. Urlaub, and J. E. Galán, Proc. Natl. Acad. Sci. USA 95:2574-2579, 1998; M. W. Wood, R. Rosqvist, P. B. Mullan, M. H. Edwards, and E. E. Galyov, Mol. Microbiol. 22:327-338, 1996). In this study, we provide evidence that an unlinked gene carried within the Salmonella pathogenicity island 1 (SPI-1), invB (K. Eichelberg, C. Ginocchio, and J. E. Galán, J. Bacteriol. 176:4501-4510, 1994), is required for the secretion of SopE through the SPI-1 TTSS. Furthermore, far-Western blotting analysis shows that SopE directly interacts with InvB through a domain located at its amino terminus. We conclude that InvB is the TTSS-associated chaperone for SopE.
摘要
SopE是鼠伤寒沙门氏菌的一种噬菌体编码效应蛋白，它通过III型分泌系统（TTSS）转运到真核细胞的细胞质中（W.-D. 哈特、H. 乌尔劳布和J. E. 加兰，《美国国家科学院院刊》95:2574 - 2579，1998；M. W. 伍德、R. 罗斯奎斯特、P. B. 穆兰、M. H. 爱德华兹和E. E. 加利奥夫，《分子微生物学》22:327 - 338，1996）。在本研究中，我们提供证据表明，沙门氏菌致病岛1（SPI - 1）中携带的一个不连锁基因invB（K. 艾歇尔贝格、C. 吉诺基奥和J. E. 加兰，《细菌学杂志》176:4501 - 4510，1994）是SopE通过SPI - 1 TTSS分泌所必需的。此外，远缘Western印迹分析表明，SopE通过位于其氨基末端的一个结构域与InvB直接相互作用。我们得出结论，InvB是SopE的TTSS相关伴侣蛋白。
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