未修饰的丙型肝炎病毒包膜糖蛋白编码序列的表达导致隐蔽内含子切除以及包含全长和部分缺失E1的E1/E2异二聚体在细胞表面的表达。
Expression of unmodified hepatitis C virus envelope glycoprotein-coding sequences leads to cryptic intron excision and cell surface expression of E1/E2 heterodimers comprising full-length and partially deleted E1.
作者信息
Dumonceaux Julie, Cormier Emmanuel G, Kajumo Francis, Donovan Gerald P, Roy-Chowdhury Jayanta, Fox Ira J, Gardner Jason P, Dragic Tatjana
机构信息
Albert Einstein College of Medicine, Bronx, New York 10461, USA.
出版信息
J Virol. 2003 Dec;77(24):13418-24. doi: 10.1128/jvi.77.24.13418-13424.2003.
Abstract
Hepatitis C virus (HCV) is a positive-strand RNA virus that replicates exclusively in the cytoplasm of infected cells. The viral envelope glycoproteins, E1 and E2, appear to be retained in the endoplasmic reticulum, where viral budding is thought to occur. Surprisingly, we found that the expression system used to generate HCV envelope glycoproteins influences their subcellular localization and processing. These findings have important implications for optimizing novel HCV fusion and entry assays as well as for budding and virus particle formation.
摘要
丙型肝炎病毒（HCV）是一种正链RNA病毒，仅在受感染细胞的细胞质中复制。病毒包膜糖蛋白E1和E2似乎保留在内质网中，据认为病毒出芽发生在此处。令人惊讶的是，我们发现用于产生HCV包膜糖蛋白的表达系统会影响它们的亚细胞定位和加工。这些发现对于优化新型HCV融合和进入检测以及出芽和病毒颗粒形成具有重要意义。
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