与鼠γ疱疹病毒68病毒粒子相关的蛋白质的鉴定
Identification of proteins associated with murine gammaherpesvirus 68 virions.
作者信息
Bortz Eric, Whitelegge Julian P, Jia Qingmei, Zhou Z Hong, Stewart James P, Wu Ting-Ting, Sun Ren
机构信息
Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095, USA.
出版信息
J Virol. 2003 Dec;77(24):13425-32. doi: 10.1128/jvi.77.24.13425-13432.2003.
Abstract
Murine gammaherpesvirus 68 (MHV68 [also known as gammaHV-68]) is distinguished by its ability to replicate to high titers in cultured cells, making it an excellent candidate for studying gammaherpesvirus virion composition. Extracellular MHV68 virions were isolated, and abundant virion-associated proteins were identified by mass spectrometry. Five nucleocapsid protein homologues, the tegument protein homologue encoded by open reading frame (ORF) 75c, and envelope glycoproteins B and H were detected. In addition, gene products from MHV68 ORF20, ORF24, ORF28, ORF45, ORF48, and ORF52 were identified in association with virions, suggesting that these gammaherpesvirus genes are involved in the early phase of infection or virion assembly and egress.
摘要
鼠γ疱疹病毒68（MHV68 [也称为γHV-68]）的特点是能够在培养细胞中复制到高滴度，这使其成为研究γ疱疹病毒病毒粒子组成的极佳候选对象。分离出细胞外的MHV68病毒粒子，并通过质谱鉴定了丰富的病毒粒子相关蛋白。检测到五个核衣壳蛋白同源物、由开放阅读框（ORF）75c编码的被膜蛋白同源物以及包膜糖蛋白B和H。此外，还鉴定出与病毒粒子相关的来自MHV68 ORF20、ORF24、ORF28、ORF45、ORF48和ORF52的基因产物，这表明这些γ疱疹病毒基因参与感染的早期阶段或病毒粒子的组装与释放。
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