Purification and characterization of recombinant Mortierella vinacea alpha-galactosidases I and II expressed in Saccharomyces cerevisiae.
作者信息
Shibuya H, Kobayashi H, Yoshida S, Kaneko S, Park G G, Kusakabe I
机构信息
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
出版信息
Biosci Biotechnol Biochem. 1999 Jun;63(6):1096-9. doi: 10.1271/bbb.63.1096.
PMID:10427697
Abstract
The cDNAs coding for Mortierella vinacea alpha-galactosidases I and II were expressed in Saccharomyces cerevisiae under the control of the yeast GAL10 promoter. The recombinant enzymes purified to homogeneity from the culture filtrate were glycosylated, and had properties identical to those of the native enzymes except for improving the heat stability of alpha-galactosidase II and decreasing the specific activities of both enzymes.
摘要
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