Natsume Ryo, Takeshita Ryo, Sugiyama Masakazu, Ohnishi Yasuo, Senda Toshiya, Horinouchi Sueharu
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2313-5. doi: 10.1107/s0907444903020766. Epub 2003 Nov 27.
CprB, an autoregulator-receptor protein from Streptomyces coelicolor A3(2), was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 6000 as a precipitant. Three crystal forms (I, II and III) were obtained; crystal forms I and II were useful for structure determination. Form I crystals belong to an orthorhombic system with space group P2(1)2(1)2(1) and diffracted to better than 2.4 A. Form II crystals belong to a tetragonal system with space group P4(1(3))2(1)2.
CprB是来自天蓝色链霉菌A3(2)的一种自调节受体蛋白，采用悬滴气相扩散法，以聚乙二醇6000作为沉淀剂进行结晶。获得了三种晶体形式（I、II和III）；晶体形式I和II可用于结构测定。I型晶体属于正交晶系，空间群为P2(1)2(1)2(1)，衍射分辨率优于2.4 Å。II型晶体属于四方晶系，空间群为P4(1(3))2(1)2。
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