Van der Zee E A, Compaan J C, de Boer M, Luiten P G
Department of Animal Physiology, University of Groningen, Haren, The Netherlands.
J Neurosci. 1992 Dec;12(12):4808-15. doi: 10.1523/JNEUROSCI.12-12-04808.1992.
In the present study, we examined changes in immunoreactivity (ir) for the gamma-isoform of protein kinase C (PKC gamma) in mouse hippocampus in relation to spatial memory processes employing the monoclonal antibody 36G9 raised against purified PKC gamma. Learning and memory were assessed by performance in a free-choice spatial pattern paradigm in a hole board in which the animals learned the pattern of 4 baited holes out of 16 holes. Adult male house mice were used, divided in four groups. Three control groups were formed: group N, naive (blank controls); group H, habituated (animals were for 5 consecutive days introduced to the hole board with all holes baited); and group PT, pseudotrained (animals were for 13 consecutive days introduced to the hole board with all holes baited). The T (trained) group was for 5 consecutive days introduced to the hole board with all holes baited (similar to the H and PT groups) followed by 8 successive days with only four holes baited in a fixed pattern. Behaviorally, following the first 5 d, the PT group crossed the hole board randomly, whereas the T group gradually learned to orientate in the hole board. The mice were killed 24 hr after the last performance. A shift in 36G9-ir appeared from the cell somata to the dendrites of hippocampal principal neurons when comparing the H and PT group, respectively. In contrast, the T group showed strong PKC gamma-ir in both cell somata and dendrites, which clearly exceeded that of the H and PT mice. In this way, 36G9-ir reveals the physiologically activated neurons involved in hole board learning.(ABSTRACT TRUNCATED AT 250 WORDS)
在本研究中，我们使用针对纯化的蛋白激酶Cγ（PKCγ）产生的单克隆抗体36G9，检测了小鼠海马体中PKCγγ亚型免疫反应性（ir）的变化与空间记忆过程的关系。通过在一个孔板中的自由选择空间模式范式中的表现来评估学习和记忆，在该范式中动物要学习16个孔中4个有诱饵孔的模式。使用成年雄性家鼠，分为四组。形成了三个对照组：N组，未处理组（空白对照）；H组，习惯组（动物连续5天被放入所有孔都有诱饵的孔板中）；PT组，假训练组（动物连续13天被放入所有孔都有诱饵的孔板中）。T（训练）组连续5天被放入所有孔都有诱饵的孔板中（类似于H组和PT组），随后8天只有四个孔按固定模式有诱饵。行为上，在前5天之后，PT组随机穿过孔板，而T组逐渐学会在孔板中定向。在最后一次表现后24小时处死小鼠。分别比较H组和PT组时，36G9-ir从海马体主要神经元的细胞体转移到了树突。相反，T组在细胞体和树突中均显示出强烈的PKCγ-ir，明显超过H组和PT组小鼠。通过这种方式，36G9-ir揭示了参与孔板学习的生理激活神经元。（摘要截断于250字）
