Gerisch Günther, Faix Jan, Köhler Jana, Müller-Taubenberger Annette
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Cell Motil Cytoskeleton. 2004 Jan;57(1):18-25. doi: 10.1002/cm.10150.
While studying mitosis in Dictyostelium mutants with deficiencies in actin-binding proteins, we found that two of these proteins, cortexillin and Aip1, are required for the precise segregation of chromosomes. Atypical spindles and nuclei with varying DNA content indicate that mutants lacking cortexillin or Aip1 are genetically unstable. These aberrations are caused by the detachment and irregular reattachment of centrosomes to the nuclear surface. Live imaging showed how coalescing mitotic complexes give rise to a multipolar spindle, and how excess centrosomes can be eliminated by mitotic cleavage between anucleate and nucleated portions of a cell. We hypothesize that mutations in regulatory proteins of the actin network might be one cause of genetic instability of malignant tumor cells.
在研究缺乏肌动蛋白结合蛋白的盘基网柄菌突变体的有丝分裂时，我们发现其中两种蛋白，即皮层肌动蛋白和Aip1，是染色体精确分离所必需的。具有不同DNA含量的非典型纺锤体和细胞核表明，缺乏皮层肌动蛋白或Aip1的突变体在遗传上是不稳定的。这些畸变是由中心体与核表面的分离和不规则重新附着引起的。实时成像显示了合并的有丝分裂复合物如何产生多极纺锤体，以及多余的中心体如何通过细胞无核部分和有核部分之间的有丝分裂分裂而被消除。我们推测，肌动蛋白网络调节蛋白的突变可能是恶性肿瘤细胞遗传不稳定的一个原因。
