Cell Dynamics Group, Max Planck Institute of Biochemistry, am Klopferspitz 18, D-82152 Martinsried, Germany.
Cells. 2021 Aug 29;10(9):2240. doi: 10.3390/cells10092240.
Aberrant centrosome activities in mutants of result in anomalies of mitotic spindles that affect the reliability of chromosome segregation. Genetic instabilities caused by these deficiencies are tolerated in multinucleate cells, which can be produced by electric-pulse induced cell fusion as a source for aberrations in the mitotic apparatus of the mutant cells. Dual-color fluorescence labeling of the microtubule system and the chromosomes in live cells revealed the variability of spindle arrangements, of centrosome-nuclear interactions, and of chromosome segregation in the atypical mitoses observed.
在 突变体中，中心体活动异常导致有丝分裂纺锤体的异常，从而影响染色体分离的可靠性。这些缺陷引起的遗传不稳定性在多核细胞中是可以容忍的，多核细胞可以通过电脉冲诱导的细胞融合产生，作为突变细胞有丝分裂装置异常的来源。活细胞中微管系统和染色体的双色荧光标记揭示了观察到的非典型有丝分裂中纺锤体排列、中心体-核相互作用和染色体分离的可变性。
