Fackrell H B, Wiseman G M
J Gen Microbiol. 1976 Jan;92(1):1-10. doi: 10.1099/00221287-92-1-1.
The gamma haemolysin of Staphylococcus aureus 'Smith 5R' was produced on Dolman-Wilson agar overlain with cellophane. Maximal yields of crude lysin with titres of 2000 to 4000 haemolytic units/ml were obtained within 24 h at 37 degrees C in 10% (v/v) CO2 in air, on medium adjusted to pH 7-0. The crude lysin was purified 2700-fold (with 75% recovery) by ultrafiltration, gel filtration and ammonium sulphate fractionation. The specific activity of the lysin was 10(5) haemolytic units/mg protein after the dialysed active precipitate was extracted with NaCl and reprecipitated with ammonium sulphate. Purified gamma lysin was homogeneous by disc electrophoresis and immunoelectrophoresis.
金黄色葡萄球菌“史密斯5R”的γ溶血素是在覆盖有玻璃纸的多尔曼 - 威尔逊琼脂上产生的。在37℃、空气含10%（v/v）二氧化碳、pH值调至7.0的培养基中，24小时内可获得效价为2000至4000溶血单位/毫升的粗溶素最大产量。通过超滤、凝胶过滤和硫酸铵分级分离，粗溶素被纯化了2700倍（回收率75%）。用氯化钠提取透析后的活性沉淀物并用硫酸铵再沉淀后，溶素的比活性为10⁵溶血单位/毫克蛋白质。纯化的γ溶血素通过圆盘电泳和免疫电泳显示为均一的。
