[非靶基因突变及外膜通透性降低对大肠杆菌喹诺酮耐药性和多重耐药性的影响]
[Impact of non-target gene mutations and reduced permeability of outer membrane on quinolone resistance and multiple antibiotic resistance in Escherichia coli].
作者信息
Zhao Rui-hua, Shu Ming-xing, Chen Shu-zhen
机构信息
Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
出版信息
Hunan Yi Ke Da Xue Xue Bao. 2003 Jun;28(3):263-5.
OBJECTIVE
To investigate the impact of non-target gene mutations and reduced permeability of the outer membrane on quinolone resistance and multiple antibiotic resistance (mar) in Escherichia coli.
METHODS
Several high-level quinolone resistant isolates whose strains were accompanied by concurrent resistant to several antibiotics were selected. The PCR products of marOR region were sequenced to detect the possible gene changes and then the strains' outer membrane proteins (Omps) were extracted to analyze on the constitutive profiles.
RESULTS
We found two site mutations in R410 isolate: 1,870(T-->C, Val-->Ala) and 1,879(A-->C, terminator-->Ser). The OmpF deficiency was found in all 3 resistant strains.
CONCLUSION
The increased expression of MarA induced by gene mutations in marOR and the constitutive profile changes of the Omps (OmpF deficient strains) may play some role in the quinolone resistance and multiple antibiotic resistance of Escherichia coli.
目的
研究非靶基因突变和外膜通透性降低对大肠杆菌喹诺酮耐药性和多重抗生素耐药性（mar）的影响。
方法
选择几株高水平喹诺酮耐药菌株，这些菌株同时对几种抗生素耐药。对marOR区域的PCR产物进行测序以检测可能的基因变化，然后提取菌株的外膜蛋白（Omps）以分析其组成谱。
结果
我们在R410分离株中发现了两个位点突变：1870（T→C，Val→Ala）和1879（A→C，终止子→Ser）。在所有3株耐药菌株中均发现OmpF缺陷。
结论
marOR基因突变诱导的MarA表达增加以及Omps的组成谱变化（OmpF缺陷菌株）可能在大肠杆菌的喹诺酮耐药性和多重抗生素耐药性中起一定作用。
Zotero 插件