El-Obeid Adila, Sunnuqrut Nadia, Hussain Azhar, Al-Hussein Khalid, Gutiérrez Marina I, Bhatia Kishor
King Fahad National Center for Children Cancer and Research, MBC 98-16, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
Leuk Res. 2004 Feb;28(2):133-7. doi: 10.1016/s0145-2126(03)00188-7.
Expression of VEGF and VEGFR support a role for angiogenic pathways in the pathogenesis of some hematological malignances. Our goal was to determine if expression of these angiogenic molecules also extend to childhood precursor B cell acute lymphoblastic leukemia (pre-B ALL). We now show that transcripts of VEGF, and its receptors VEGFR-1 and VEGFR-2 are concomitantly expressed in both ALL cell lines and primary pre-B ALL. Western blot and ELISA consistently detected VEGF protein in the supernatants of the cell lines. Similarly, VEGFR-1 and VEGFR-2 proteins are also detectable by FACS analysis. Interestingly, the expression of the receptors in immature B cells is limited to the intra-cytoplasmic compartment and may suggest either internalization of the receptors or a block in trafficking of the receptor to the surface.
血管内皮生长因子（VEGF）和血管内皮生长因子受体（VEGFR）的表达支持血管生成途径在某些血液系统恶性肿瘤发病机制中的作用。我们的目标是确定这些血管生成分子的表达是否也存在于儿童前体B细胞急性淋巴细胞白血病（pre - B ALL）中。我们现在表明，VEGF及其受体VEGFR - 1和VEGFR - 2的转录本在ALL细胞系和原发性pre - B ALL中均同时表达。蛋白质免疫印迹法和酶联免疫吸附测定法一致检测到细胞系上清液中的VEGF蛋白。同样，通过流式细胞术分析也可检测到VEGFR - 1和VEGFR - 2蛋白。有趣的是，未成熟B细胞中受体的表达仅限于细胞质内区室，这可能表明受体发生了内化作用或受体向表面转运过程受阻。
