Dorange J L, Delaforge M, Janiaud P, Padieu P
C R Seances Soc Biol Fil. 1977;171(5):1041-8.
Mutagenicity of the metabolites of the expoxide-diol pathway of safrole and analogues was studied on Ames' strains with Ames' method. Safrole, eugenol, eugenolmethylether, estragol, allylbenzene and 1'-hydroxysafrole, are not mutagen on TA 1535, TA 100 (point mutation) and TA 1537, TA 1538, TA 98 (frameshift mutations) without activation system. The corresponding epoxides that we have synthetized, are mutagens and inducers of point mutation in TA 1535 and TA 100. Dose-effect curves show differences between the mutagen efficiencies of these epoxides probably in relation with their electrophilic properties. On the other hand the 2', 3'-dihydro-2',3'-dihydroxisafrole was not mutagen in Ames' test. These results confirm the promutagen character of safrole and analogues and the role of the epoxides as proximate carcinogens.
采用艾姆斯试验方法，在艾姆斯菌株上研究了黄樟素及其类似物的环氧化物 - 二醇途径代谢产物的致突变性。黄樟素、丁香酚、丁香酚甲醚、草蒿脑、烯丙基苯和1'-羟基黄樟素，在没有活化系统的情况下，对TA 1535、TA 100（点突变）以及TA 1537、TA 1538、TA 98（移码突变）菌株均无致突变性。我们合成的相应环氧化物是TA 1535和TA 100中致点突变的诱变剂和诱导剂。剂量 - 效应曲线显示这些环氧化物诱变效率之间存在差异，这可能与它们的亲电性质有关。另一方面，2', 3'-二氢 - 2',3'-二羟基黄樟素在艾姆斯试验中无致突变性。这些结果证实了黄樟素及其类似物的前诱变特性以及环氧化物作为近端致癌物的作用。
