Green N R, Savage J R
Mutat Res. 1978 May;57(2):115-21. doi: 10.1016/0027-5107(78)90257-9.
The mutagenicity of safrole, eugenol, the secondary amines, with which they combine during metabolism, and the ninhydrin positive urinary metabolites of safrole and eugenol was tested. The panel of tests included the direct bacterial assay, a microsomal mutagenesis assay and a host-mediated assay. With the direct bacterial assay employing four mutant strains of Salmonella typhimurium (TA1530, TA1531, TA1532, TA1964), all the compounds gave negative results. In the microsomal mutagenesis assay, employing the same four mutant strains, safrole and safrole metabolite II were mutagenic with strains TA1530 and TA1532. Dimethylamine was also found to be a weak mutagen in the microsomal mutagenesis assay with strain TA1530. Safrole and safrole metabolite II were also mutagenic in the host-mediated assay with strains TA1950 and TA1952. Negative results were observed for safrole metabolites I and III, eugenol, eugenol metabolites I and II, piperidine, pipecolic acid, proline, and pyrrolidine in all three assay systems.
测试了黄樟素、丁香酚、它们在代谢过程中与之结合的仲胺以及黄樟素和丁香酚的茚三酮阳性尿代谢物的致突变性。测试项目包括直接细菌试验、微粒体诱变试验和宿主介导试验。在使用四株鼠伤寒沙门氏菌突变株（TA1530、TA1531、TA1532、TA1964）的直接细菌试验中，所有化合物均给出阴性结果。在使用相同四株突变株的微粒体诱变试验中，黄樟素和黄樟素代谢物II对TA1530和TA1532菌株具有致突变性。在使用TA1530菌株的微粒体诱变试验中，二甲胺也被发现是一种弱诱变剂。在使用TA1950和TA1952菌株的宿主介导试验中，黄樟素和黄樟素代谢物II也具有致突变性。在所有三种试验系统中，黄樟素代谢物I和III、丁香酚、丁香酚代谢物I和II、哌啶、哌可酸、脯氨酸和吡咯烷均给出阴性结果。
