Jessberger Sebastian, Kempermann Gerd
VolkswagenStiftung Research Group at the Department of Experimental Neurology, Charité University Hospital, Humboldt University Berlin, Robert-Rössle-Str. 10, D-13125 Berlin, Berlin-Buch, Germany.
Eur J Neurosci. 2003 Nov;18(10):2707-12. doi: 10.1111/j.1460-9568.2003.02986.x.
The mammalian hippocampus is a highly plastic brain structure in which new neurons are generated throughout adulthood. Do these new neurons qualitatively and quantitatively participate in the activity-dependent gene expression after challenging the hippocampus in a learning task? Adult mice were injected with bromodeoxyuridine (BrdU) to label dividing cells and indeed, individual BrdU-labelled new neurons expressed the immediate early gene protein (IEGp) c-fos during the acquisition phase of the Morris water maze task to the same extend as older granule cells. To assess the responsiveness of all new neurons we also analysed the expression patterns of three IEGps (c-fos, zif268 and Homer1A) after kainic acid (KA)- or pentylenetetrazol (PTZ)-induced seizures. We found that after a maturation period of between 2 and 5 weeks a comparable ratio of adult-generated granule cells participated in IEGp-expression as in the population of older granule cells. Thus, new neurons appear quantitatively integrated into hippocampal circuits, suggestive of a relevant contribution to hippocampal function.
哺乳动物的海马体是一种高度可塑性的脑结构，在成年期会持续产生新的神经元。在学习任务中对海马体进行刺激后，这些新神经元在质量和数量上是否参与了依赖活动的基因表达？给成年小鼠注射溴脱氧尿苷（BrdU）以标记分裂细胞，实际上，在莫里斯水迷宫任务的获取阶段，单个BrdU标记的新神经元与较老的颗粒细胞一样，表达即刻早期基因蛋白（IEGp）c-fos。为了评估所有新神经元的反应性，我们还分析了在 kainic acid（KA）或戊四氮（PTZ）诱导的癫痫发作后三种IEGp（c-fos、zif268和Homer1A）的表达模式。我们发现，经过2至5周的成熟期后，成年产生的颗粒细胞参与IEGp表达的比例与较老颗粒细胞群体中的比例相当。因此，新神经元似乎在数量上整合到海马回路中，这表明它们对海马功能有相关贡献。
