采用流动注射技术结合固相萃取法测定波吲洛尔。

Determination of bopindolol using the flow injection technique coupled with solid phase extraction.

作者信息

Meireles Joana, Sklenárová Hana, Satínský Dalibor, Solich Petr, Araújo Alberto N, Montenegro Maria Conceição B S M

机构信息

CEQUP/Department of Physical Chemistry, Faculty of Pharmacy, University of Porto, Rua Aníbal Cunha 164, 4050, Porto, Portugal.

出版信息

J Pharm Biomed Anal. 2003 Dec 4;33(5):1149-53. doi: 10.1016/s0731-7085(03)00430-8.

DOI:10.1016/s0731-7085(03)00430-8

PMID:14656606

Abstract

In the proposed procedure, the determination of bopindolol using a flow injection analysis (FIA) technique, with spectrophotometric detection at 635 nm, is described. The method is based on the production of a green, water-soluble complex with ferric ions in acid medium. The automated lab-made FIA system was used for the direct determination of bopindolol in tablets. Bopindolol was adsorbed onto the solid phase in a mini-column, which was integrated directly into the flow system. The positive feature of the use of solid phase extraction (SPE) was the pre-concentration of bopindolol (seven times). The sample throughput was 50 samples per hour. Using the SPE method, bopindolol was determined with a linear range from 125 to 1000 microg ml(-1) (Relative standard deviation (R.S.D.)=1.87%), with a detection limit (3sigma) of 70 microg ml(-1). The method was applied to the determination of bopindolol in Sandonorm tablets. The results obtained were compared with a conventional HPLC method, both analytical techniques were in good agreement.

摘要

本文描述了一种采用流动注射分析（FIA）技术、在635nm处进行分光光度检测来测定波吲洛尔的方法。该方法基于在酸性介质中波吲洛尔与铁离子形成绿色水溶性络合物。使用实验室自制的自动化FIA系统直接测定片剂中的波吲洛尔。波吲洛尔被吸附到微型柱中的固定相上，该微型柱直接集成到流动系统中。使用固相萃取（SPE）的积极特点是波吲洛尔可预富集（七倍）。样品通量为每小时50个样品。采用SPE方法测定波吲洛尔，线性范围为125至1000μg ml⁻¹（相对标准偏差（R.S.D.）=1.87%），检测限（3σ）为70μg ml⁻¹。该方法应用于测定桑多洛尔片剂中的波吲洛尔。将所得结果与传统的HPLC方法进行比较，两种分析技术结果吻合良好。