Ren Chengwei, Lee Mi-Kyung, Yan Bo, Ding Kejiao, Cox Bettye, Romanov Michael N, Price Jennifer A, Dodgson Jerry B, Zhang Hong-Bin
Department of Soil and Crop Sciences and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, Texas 77843, USA.
Genome Res. 2003 Dec;13(12):2754-8. doi: 10.1101/gr.1499303.
A genome-wide physical map constructed with bacterial artificial chromosomes (BACs) is an essential component in linking phenotypic traits to the responsible genetic variation in the genomes of plants and animals. We have constructed a physical map of the chicken genome from 57,091 BACs (7.9-fold haploid genome coverage) by restriction fingerprint analysis using high-resolution polyacrylamide gel electrophoresis. The physical map consists of 2331 overlapping BAC contigs and is estimated to span 1510 Mb in physical length. BAC contigs were verified manually and by screening the BACs with 367 DNA markers. A total of 361 of the contigs have been anchored to the existing chicken genetic map. This map represents the first genome-wide, BAC-based physical map of the chicken genome. It provides a powerful platform for many areas of chicken genomics, including targeted marker development, fine mapping of genes and QTL alleles, positional cloning, analysis of avian genome organization and evolution, chicken-mammalian comparative genomics, and large-scale genome sequencing.
利用细菌人工染色体(BAC)构建的全基因组物理图谱是将植物和动物基因组中的表型性状与相关遗传变异联系起来的重要组成部分。我们通过使用高分辨率聚丙烯酰胺凝胶电泳的限制性指纹分析,从57,091个BAC(单倍体基因组覆盖度为7.9倍)构建了鸡基因组的物理图谱。该物理图谱由2331个重叠的BAC重叠群组成,估计物理长度跨越1510 Mb。BAC重叠群通过人工验证以及用367个DNA标记筛选BAC进行了验证。共有361个重叠群已被锚定到现有的鸡遗传图谱上。该图谱代表了首张全基因组的、基于BAC的鸡基因组物理图谱。它为鸡基因组学的许多领域提供了一个强大的平台,包括靶向标记开发、基因和QTL等位基因的精细定位、定位克隆、鸟类基因组组织和进化分析、鸡 - 哺乳动物比较基因组学以及大规模基因组测序。