人类基因组的物理图谱。

A physical map of the human genome.

作者信息

McPherson J D, Marra M, Hillier L, Waterston R H, Chinwalla A, Wallis J, Sekhon M, Wylie K, Mardis E R, Wilson R K, Fulton R, Kucaba T A, Wagner-McPherson C, Barbazuk W B, Gregory S G, Humphray S J, French L, Evans R S, Bethel G, Whittaker A, Holden J L, McCann O T, Dunham A, Soderlund C, Scott C E, Bentley D R, Schuler G, Chen H C, Jang W, Green E D, Idol J R, Maduro V V, Montgomery K T, Lee E, Miller A, Emerling S, Gibbs R, Scherer S, Gorrell J H, Sodergren E, Clerc-Blankenburg K, Tabor P, Naylor S, Garcia D, de Jong P J, Catanese J J, Nowak N, Osoegawa K, Qin S, Rowen L, Madan A, Dors M, Hood L, Trask B, Friedman C, Massa H, Cheung V G, Kirsch I R, Reid T, Yonescu R, Weissenbach J, Bruls T, Heilig R, Branscomb E, Olsen A, Doggett N, Cheng J F, Hawkins T, Myers R M, Shang J, Ramirez L, Schmutz J, Velasquez O, Dixon K, Stone N E, Cox D R, Haussler D, Kent W J, Furey T, Rogic S, Kennedy S, Jones S, Rosenthal A, Wen G, Schilhabel M, Gloeckner G, Nyakatura G, Siebert R, Schlegelberger B, Korenberg J, Chen X N, Fujiyama A, Hattori M, Toyoda A, Yada T, Park H S, Sakaki Y, Shimizu N, Asakawa S, Kawasaki K, Sasaki T, Shintani A, Shimizu A, Shibuya K, Kudoh J, Minoshima S, Ramser J, Seranski P, Hoff C, Poustka A, Reinhardt R, Lehrach H

机构信息

Washington University School of Medicine, Genome Sequencing Center, Department of Genetics, St. Louis, Missouri 63108, USA.

出版信息

Nature. 2001 Feb 15;409(6822):934-41. doi: 10.1038/35057157.

DOI:10.1038/35057157

PMID:11237014

Abstract

The human genome is by far the largest genome to be sequenced, and its size and complexity present many challenges for sequence assembly. The International Human Genome Sequencing Consortium constructed a map of the whole genome to enable the selection of clones for sequencing and for the accurate assembly of the genome sequence. Here we report the construction of the whole-genome bacterial artificial chromosome (BAC) map and its integration with previous landmark maps and information from mapping efforts focused on specific chromosomal regions. We also describe the integration of sequence data with the map.

摘要

人类基因组是迄今为止已测序的最大基因组，其大小和复杂性给序列组装带来了诸多挑战。国际人类基因组测序联盟构建了全基因组图谱，以便选择用于测序的克隆，并实现基因组序列的精确组装。在此，我们报告全基因组细菌人工染色体（BAC）图谱的构建，以及它与先前的标志性图谱和专注于特定染色体区域的定位研究信息的整合。我们还描述了序列数据与该图谱的整合。