Carlson Anne E, Westenbroek Ruth E, Quill Timothy, Ren Dejian, Clapham David E, Hille Bertil, Garbers David L, Babcock Donner F
Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA.
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14864-8. doi: 10.1073/pnas.2536658100. Epub 2003 Dec 1.
CatSper family proteins are putative ion channels expressed exclusively in membranes of the sperm flagellum and required for male fertility. Here, we show that mouse CatSper1 is essential for depolarization-evoked Ca2+ entry and for hyperactivated movement, a key flagellar function. CatSper1 is not needed for other developmental landmarks, including regional distributions of CaV1.2, CaV2.2, and CaV2.3 ion channel proteins, the cAMP-mediated activation of motility by HCO3-, and the protein phosphorylation cascade of sperm capacitation. We propose that CatSper1 functions as a voltage-gated Ca2+ channel that controls Ca2+ entry to mediate the hyperactivated motility needed late in the preparation of sperm for fertilization.
CatSper家族蛋白是一种推测的离子通道，仅在精子鞭毛膜中表达，是雄性生育所必需的。在这里，我们表明小鼠CatSper1对于去极化诱发的Ca2+内流和超激活运动（一种关键的鞭毛功能）至关重要。CatSper1对于其他发育标志并非必需，包括CaV1.2、CaV2.2和CaV2.3离子通道蛋白的区域分布、HCO3-介导的cAMP对运动性的激活以及精子获能的蛋白磷酸化级联反应。我们提出，CatSper1作为一种电压门控Ca2+通道发挥作用，控制Ca2+内流，以介导精子为受精做准备后期所需的超激活运动。
