McMurdy John W, Berger Andrew J
The Institute of Optics, University of Rochester, Rochester, New York 14627, USA.
Appl Spectrosc. 2003 May;57(5):522-5. doi: 10.1366/000370203321666533.
Spectroscopic methods of urinalysis offer several advantages over chemical methods, including less sample contact and higher information content. In particular, urine creatinine has been the subject of several spectroscopic studies. We report the first use of Raman spectroscopy to measure creatinine concentrations in unaltered urine samples from a multipatient population. Using near-infrared excitation and a hybrid linear analysis calibration method, a root mean squared error of cross-validation (RMSECV) of 4.9 mg/dL was obtained. The error in the reference chemical method was 1.1 mg/dL. This result shows that the Raman spectroscopy can measure creatinine at clinical levels even in the presence of patient-to-patient variations. Because most assays in urine require creatinine concentration in order to correct for fluctuations in water content, measurement of creatinine is the first step towards more extensive Raman-based urinalysis.
尿液分析的光谱方法相对于化学方法具有几个优点，包括更少的样品接触和更高的信息含量。特别是，尿肌酐一直是几项光谱研究的主题。我们报告了首次使用拉曼光谱法测量来自多患者群体的未改变尿液样本中的肌酐浓度。使用近红外激发和混合线性分析校准方法，获得了4.9mg/dL的交叉验证均方根误差（RMSECV）。参考化学方法的误差为1.1mg/dL。该结果表明，即使存在患者间差异，拉曼光谱法也能在临床水平上测量肌酐。由于尿液中的大多数检测都需要肌酐浓度来校正水分含量的波动，因此肌酐的测量是迈向更广泛的基于拉曼光谱的尿液分析的第一步。
