Department of Urology, Institute of Urology, Organ Transplantation Center, West China Hospital, Sichuan University, 37 Guoxuexiang, Chengdu, 610041, China.
Department of Urologic Sciences, University of British Columbia, 2660 Oak Street, Vancouver, BC, V6H 3Z6, Canada.
Sci Rep. 2021 Jan 28;11(1):2463. doi: 10.1038/s41598-021-82113-7.
Routine monitoring of kidney transplant function is required for the standard care in post-transplantation management, including frequent measurements of serum creatinine with or without kidney biopsy. However, the invasiveness of these methods with potential for clinically significant complications makes them less than ideal. The objective of this study was to develop a non-invasive tool to monitor the kidney transplant function by using Surface-Enhanced Raman Spectroscopy (SERS). Urine and blood samples were collected from kidney transplant recipients after surgery. Silver nanoparticle-based SERS spectra of the urine were measured and evaluated using partial least squires (PLS) analysis. The SERS spectra were compared with conventional chemical markers of kidney transplant function to assess its predictive ability. A total of 110 kidney transplant recipients were included in this study. PLS results showed significant correlation with urine protein (R = 0.4660, p < 0.01), creatinine (R = 0.8106, p < 0.01), and urea (R = 0.7808, p < 0.01). Furthermore, the prediction of the blood markers of kidney transplant function using the urine SERS spectra was indicated by R = 0.7628 (p < 0.01) for serum creatinine and R = 0.6539 (p < 0.01) for blood urea nitrogen. This preliminary study suggested that the urine SERS spectral analysis could be used as a convenient method for rapid assessment of kidney transplant function.
常规监测肾移植功能是移植后管理标准护理的要求，包括频繁测量血清肌酐，或结合肾活检。然而，这些方法具有侵入性，可能会引起临床显著的并发症，因此并不理想。本研究旨在开发一种非侵入性工具，通过表面增强拉曼光谱（SERS）来监测肾移植功能。在手术后，从肾移植受者收集尿液和血液样本。测量基于银纳米粒子的 SERS 尿液光谱，并使用偏最小二乘（PLS）分析进行评估。将 SERS 光谱与肾移植功能的常规化学标志物进行比较，以评估其预测能力。本研究共纳入 110 例肾移植受者。PLS 结果显示与尿液蛋白（R = 0.4660，p < 0.01）、肌酐（R = 0.8106，p < 0.01）和尿素（R = 0.7808，p < 0.01）呈显著相关。此外，通过尿液 SERS 光谱预测肾移植功能的血液标志物，血清肌酐的 R = 0.7628（p < 0.01），血尿素氮的 R = 0.6539（p < 0.01）。这项初步研究表明，尿液 SERS 光谱分析可作为快速评估肾移植功能的便捷方法。
