Peacock Sharon J, Justice Anita, Griffiths D, de Silva G D I, Kantzanou M N, Crook Derrick, Sleeman Karen, Day Nicholas P J
Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, United Kingdom.
J Clin Microbiol. 2003 Dec;41(12):5718-25. doi: 10.1128/JCM.41.12.5718-5725.2003.
Nasal carriage of Staphylococcus aureus is a major risk factor for invasive S. aureus disease. The aim of this study was to define factors associated with carriage. We conducted a prospective, longitudinal community-based study of infants and their mothers for a period of 6 months following delivery. The epidemiology of carriage was examined for 100 infant-mother pairs. Infant carriage varied significantly with age, falling from 40 to 50% during the first 8 weeks to 21% by 6 months. Determinants of infant S. aureus carriage included maternal carriage, breastfeeding, and number of siblings. Bacterial typing of S. aureus was performed by pulsed-field gel electrophoresis and multilocus sequence typing. The majority of individuals carried a single strain of S. aureus over time, and the mother was the usual source for colonizing isolates in infants. The effect of other components of the normal nasal flora on the development of S. aureus carriage was examined in 157 consecutive infants. Negative associations (putative bacterial interference) between S. aureus and other species occurred early in infancy but were not sustained. An increasing antistaphylococcal effect observed over time was not attributable to bacterial interference. S. aureus carriage in infants is likely to be determined by a combination of host, environmental, and bacterial factors, but bacterial interference does not appear to be an ultimate determinant of carrier status.
金黄色葡萄球菌的鼻腔携带是侵袭性金黄色葡萄球菌疾病的主要危险因素。本研究的目的是确定与携带相关的因素。我们对婴儿及其母亲进行了一项基于社区的前瞻性纵向研究，在分娩后的6个月内进行随访。对100对母婴的携带情况进行了流行病学调查。婴儿携带率随年龄有显著变化，在出生后的前8周从40%至50%降至6个月时的21%。婴儿金黄色葡萄球菌携带的决定因素包括母亲携带、母乳喂养和兄弟姐妹数量。通过脉冲场凝胶电泳和多位点序列分型对金黄色葡萄球菌进行细菌分型。随着时间的推移，大多数个体携带单一菌株的金黄色葡萄球菌，母亲是婴儿定植菌株的常见来源。在157例连续的婴儿中研究了正常鼻腔菌群的其他成分对金黄色葡萄球菌携带发展的影响。金黄色葡萄球菌与其他菌种之间的负相关（假定的细菌干扰）在婴儿早期出现，但未持续存在。随着时间的推移观察到的抗葡萄球菌作用增强并非归因于细菌干扰。婴儿金黄色葡萄球菌携带情况可能由宿主、环境和细菌因素共同决定，但细菌干扰似乎不是携带者状态的最终决定因素。
