Enright M C, Day N P, Davies C E, Peacock S J, Spratt B G
Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, Oxford University, Oxford OX1 3FY.
J Clin Microbiol. 2000 Mar;38(3):1008-15. doi: 10.1128/JCM.38.3.1008-1015.2000.
A multilocus sequence typing (MLST) scheme has been developed for Staphylococcus aureus. The sequences of internal fragments of seven housekeeping genes were obtained for 155 S. aureus isolates from patients with community-acquired and hospital-acquired invasive disease in the Oxford, United Kingdom, area. Fifty-three different allelic profiles were identified, and 17 of these were represented by at least two isolates. The MLST scheme was highly discriminatory and was validated by showing that pairs of isolates with the same allelic profile produced very similar SmaI restriction fragment patterns by pulsed-field gel electrophoresis. All 22 isolates with the most prevalent allelic profile were methicillin-resistant S. aureus (MRSA) isolates and had allelic profiles identical to that of a reference strain of the epidemic MRSA clone 16 (EMRSA-16). Four MRSA isolates that were identical in allelic profile to the other major epidemic MRSA clone prevalent in British hospitals (clone EMRSA-15) were also identified. The majority of isolates (81%) were methicillin-susceptible S. aureus (MSSA) isolates, and seven MSSA clones included five or more isolates. Three of the MSSA clones included at least five isolates from patients with community-acquired invasive disease and may represent virulent clones with an increased ability to cause disease in otherwise healthy individuals. The most prevalent MSSA clone (17 isolates) was very closely related to EMRSA-16, and the success of the latter clone at causing disease in hospitals may be due to its emergence from a virulent MSSA clone that was already a major cause of invasive disease in both the community and hospital settings. MLST provides an unambiguous method for assigning MRSA and MSSA isolates to known clones or assigning them as novel clones via the Internet.
已开发出一种用于金黄色葡萄球菌的多位点序列分型（MLST）方案。从英国牛津地区社区获得性和医院获得性侵袭性疾病患者的155株金黄色葡萄球菌分离株中，获取了7个管家基因内部片段的序列。鉴定出53种不同的等位基因谱型，其中17种谱型至少由两个分离株代表。该MLST方案具有高度鉴别力，并通过以下方式得到验证：具有相同等位基因谱型的分离株对通过脉冲场凝胶电泳产生非常相似的SmaI限制性片段模式。所有22株具有最常见等位基因谱型的分离株均为耐甲氧西林金黄色葡萄球菌（MRSA）分离株，其等位基因谱型与流行的MRSA克隆16（EMRSA - 16）的参考菌株相同。还鉴定出4株等位基因谱型与英国医院中流行的另一个主要MRSA克隆（克隆EMRSA - 15）相同的MRSA分离株。大多数分离株（81%）为甲氧西林敏感金黄色葡萄球菌（MSSA）分离株，7个MSSA克隆包含5个或更多分离株。其中3个MSSA克隆包含至少5株来自社区获得性侵袭性疾病患者的分离株，可能代表在健康个体中致病能力增强的毒性克隆。最常见的MSSA克隆（17株分离株）与EMRSA - 16密切相关，后一个克隆在医院中致病的成功可能归因于它从一个毒性MSSA克隆演变而来，而该毒性MSSA克隆在社区和医院环境中已经是侵袭性疾病的主要病因。MLST提供了一种明确的方法，可通过互联网将MRSA和MSSA分离株指定到已知克隆或指定为新克隆。
