基于多位点可变数目串联重复序列分析（MLVA）的产志贺毒素大肠杆菌O157的DNA指纹图谱分析
DNA fingerprinting of Shiga-toxin producing Escherichia coli O157 based on Multiple-Locus Variable-Number Tandem-Repeats Analysis (MLVA).
作者信息
Lindstedt Bjørn-Arne, Heir Even, Gjernes Elisabet, Vardund Traute, Kapperud Georg
机构信息
Norwegian Institute of Public Health, Department of Infectious Disease Control, Oslo, Norway.
出版信息
Ann Clin Microbiol Antimicrob. 2003 Dec 10;2:12. doi: 10.1186/1476-0711-2-12.
BACKGROUND
The ability to react early to possible outbreaks of Escherichia coli O157:H7 and to trace possible sources relies on the availability of highly discriminatory and reliable techniques. The development of methods that are fast and has the potential for complete automation is needed for this important pathogen.
METHODS
In all 73 isolates of shiga-toxin producing E. coli O157 (STEC) were used in this study. The two available fully sequenced STEC genomes were scanned for tandem repeated stretches of DNA, which were evaluated as polymorphic markers for isolate identification.
RESULTS
The 73 E. coli isolates displayed 47 distinct patterns and the MLVA assay was capable of high discrimination between the E. coli O157 strains. The assay was fast and all the steps can be automated.
CONCLUSION
The findings demonstrate a novel high discriminatory molecular typing method for the important pathogen E. coli O157 that is fast, robust and offers many advantages compared to current methods.
背景
对大肠杆菌O157:H7可能爆发疫情做出早期反应以及追踪可能来源的能力，依赖于高度区分性和可靠技术的可用性。对于这种重要病原体，需要开发快速且具有完全自动化潜力的方法。
方法
本研究共使用了73株产志贺毒素大肠杆菌O157（STEC）分离株。对两个已完成全基因组测序的STEC基因组进行扫描，寻找串联重复的DNA片段，将其评估为用于分离株鉴定的多态性标记。
结果
73株大肠杆菌分离株呈现出47种不同模式，多重荧光定量PCR分型分析能够对大肠杆菌O157菌株进行高度区分。该分析快速，所有步骤均可自动化。
结论
研究结果证明了一种针对重要病原体大肠杆菌O157的新型高区分性分子分型方法，该方法快速、稳健，与现有方法相比具有诸多优势。
Zotero 插件