通过可变数量串联重复序列分析对桑萎蔫病菌株进行鉴别
Differentiation of strains of Xylella fastidiosa by a variable number of tandem repeat analysis.
作者信息
Coletta-Filho H D, Takita M A, de Souza A A, Aguilar-Vildoso C I, Machado M A
机构信息
Centro de Citricultura Sylvio Moreira, Instituto Agronômico, CEP 13490-970, Cordeirópolis, São Paulo, Brazil.
出版信息
Appl Environ Microbiol. 2001 Sep;67(9):4091-5. doi: 10.1128/AEM.67.9.4091-4095.2001.
Abstract
Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen Xylella fastidiosa and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, which we examined for allelic polymorphisms using PCR. Five genuine VNTR loci were highly polymorphic within a set of 27 X. fastidiosa strains from different hosts. The highest average Nei's measure of genetic diversity (H) estimated for VNTR loci was 0.51, compared to 0.17 derived from randomly amplified polymorphic DNA (RAPD) analysis. For citrus X. fastidiosa strains, some specific VNTR loci had a H value of 0.83, while the maximum value given by specific RAPD loci was 0.12. Our approach using VNTR markers provides a high-resolution tool for epidemiological, genetic, and ecological analysis of citrus-specific X. fastidiosa strains.
摘要
在柑橘病原体木质部难养菌的基因组中鉴定出具有潜在可变串联重复序列（VNTR）位点的短串联重复序列（SSR），并将其用于分型研究。虽然不存在单核苷酸和二核苷酸重复序列，但我们发现了几个中等长度的7、8和9核苷酸重复序列，我们使用聚合酶链反应（PCR）对其等位基因多态性进行了检测。在一组来自不同宿主的27株木质部难养菌中，5个真正的VNTR位点具有高度多态性。VNTR位点估计的最高平均奈氏遗传多样性指数（H）为0.51，而随机扩增多态性DNA（RAPD）分析得出的该指数为0.17。对于柑橘木质部难养菌菌株，一些特定的VNTR位点的H值为0.83，而特定RAPD位点的最大值为0.12。我们使用VNTR标记的方法为柑橘特异性木质部难养菌菌株的流行病学、遗传学和生态学分析提供了一种高分辨率工具。
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本文引用的文献
1
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Plant Dis. 1998 Jan;82(1):94-97. doi: 10.1094/PDIS.1998.82.1.94.
2
Distribution of Xylella fastidiosa in Oaks in Florida and Its Association with Growth Decline in Quercus laevis.佛罗里达州栎树中桑氏木质部小菌的分布及其与平滑栎生长衰退的关系
Plant Dis. 1998 May;82(5):569-572. doi: 10.1094/PDIS.1998.82.5.569.
3
Culture and serological detection of the xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain ofXylella fastidiosa.木质部限制作物黄化脉明细菌的培养和血清学检测及其鉴定为木质部难养细菌的一个菌株。
Curr Microbiol. 1993 Sep;27(3):137-42. doi: 10.1007/BF01576010.
4
Fastidious xylem-limited bacterial plant pathogens.苛求的木质部受限细菌植物病原体。
Annu Rev Phytopathol. 1996;34:131-51. doi: 10.1146/annurev.phyto.34.1.131.
5
Genetic diversity of Pierce's disease strains and other pathotypes of Xylella fastidiosa.皮尔斯病病菌株及其他桑萎蔫病菌致病型的遗传多样性。
Appl Environ Microbiol. 2001 Feb;67(2):895-903. doi: 10.1128/AEM.67.2.895-903.2001.
6
Genetic diversity of Mycobacterium africanum clinical isolates based on IS6110-restriction fragment length polymorphism analysis, spoligotyping, and variable number of tandem DNA repeats.基于IS6110-限制性片段长度多态性分析、间隔寡核苷酸分型及可变数目串联重复序列的非洲分枝杆菌临床分离株的遗传多样性
J Clin Microbiol. 2001 Jan;39(1):57-65. doi: 10.1128/JCM.39.1.57-65.2001.
7
The genome sequence of the plant pathogen Xylella fastidiosa. The Xylella fastidiosa Consortium of the Organization for Nucleotide Sequencing and Analysis.植物病原体桑萎蔫病菌的基因组序列。核苷酸测序与分析组织的桑萎蔫病菌联盟。
Nature. 2000 Jul 13;406(6792):151-9. doi: 10.1038/35018003.
8
Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis.多位点可变数目串联重复序列分析揭示了炭疽芽孢杆菌内部的遗传关系。
J Bacteriol. 2000 May;182(10):2928-36. doi: 10.1128/JB.182.10.2928-2936.2000.
9
Diversity in a variable-number tandem repeat from Yersinia pestis.鼠疫耶尔森氏菌可变数目串联重复序列的多样性。
J Clin Microbiol. 2000 Apr;38(4):1516-9. doi: 10.1128/JCM.38.4.1516-1519.2000.
10
Occurrence and structure-function relationship of pentameric short sequence repeats in microbial genomes.微生物基因组中五聚体短序列重复序列的发生及其结构-功能关系
Res Microbiol. 1999 Nov-Dec;150(9-10):617-26. doi: 10.1016/s0923-2508(99)00129-1.
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