Jermstad Kathleen D, Bassoni Daniel L, Jech Keith S, Ritchie Gary A, Wheeler Nicholas C, Neale David B
Institute of Forest Genetics, Pacific Southwest Research Station, U.S. Department of Agriculture Forest Service, Placerville, California 95667, USA.
Genetics. 2003 Nov;165(3):1489-506. doi: 10.1093/genetics/165.3.1489.
Quantitative trait loci (QTL) were mapped in the woody perennial Douglas fir (Pseudotsuga menziesii var. menziesii [Mirb.] Franco) for complex traits controlling the timing of growth initiation and growth cessation. QTL were estimated under controlled environmental conditions to identify QTL interactions with photoperiod, moisture stress, winter chilling, and spring temperatures. A three-generation mapping population of 460 cloned progeny was used for genetic mapping and phenotypic evaluations. An all-marker interval mapping method was used for scanning the genome for the presence of QTL and single-factor ANOVA was used for estimating QTL-by-environment interactions. A modest number of QTL were detected per trait, with individual QTL explaining up to 9.5% of the phenotypic variation. Two QTL-by-treatment interactions were found for growth initiation, whereas several QTL-by-treatment interactions were detected among growth cessation traits. This is the first report of QTL interactions with specific environmental signals in forest trees and will assist in the identification of candidate genes controlling these important adaptive traits in perennial plants.
对木本多年生植物花旗松（Pseudotsuga menziesii var. menziesii [Mirb.] Franco）中控制生长开始和停止时间的复杂性状进行了数量性状位点（QTL）定位。在可控环境条件下估计QTL，以识别QTL与光周期、水分胁迫、冬季低温和春季温度的相互作用。利用一个由460个克隆后代组成的三代作图群体进行遗传作图和表型评估。采用全标记区间作图法扫描基因组中QTL的存在情况，并采用单因素方差分析估计QTL与环境的相互作用。每个性状检测到数量不多的QTL，单个QTL解释的表型变异高达9.5%。在生长开始方面发现了两个QTL与处理的相互作用，而在生长停止性状中检测到了几个QTL与处理的相互作用。这是林木中QTL与特定环境信号相互作用的首次报道，将有助于鉴定控制多年生植物这些重要适应性性状的候选基因。
