Brown Garth R, Bassoni Daniel L, Gill Geoffrey P, Fontana Joseph R, Wheeler Nicholas C, Megraw Robert A, Davis Mark F, Sewell Mitchell M, Tuskan Gerald A, Neale David B
Department of Environmental Horticulture, University of California-Davis, 1 Shields Avenue, Davis, CA 95616, USA.
Genetics. 2003 Aug;164(4):1537-46. doi: 10.1093/genetics/164.4.1537.
A long-term series of experiments to map QTL influencing wood property traits in loblolly pine has been completed. These experiments were designed to identify and subsequently verify QTL in multiple genetic backgrounds, environments, and growing seasons. Verification of QTL is necessary to substantiate a biological basis for observed marker-trait associations, to provide precise estimates of the magnitude of QTL effects, and to predict QTL expression at a given age or in a particular environment. Verification was based on the repeated detection of QTL among populations, as well as among multiple growing seasons for each population. Temporal stability of QTL was moderate, with approximately half being detected in multiple seasons. Fewer QTL were common to different populations, but the results are nonetheless encouraging for restricted applications of marker-assisted selection. QTL from larger populations accounted for less phenotypic variation than QTL detected in smaller populations, emphasizing the need for experiments employing much larger families. Additionally, 18 candidate genes related to lignin biosynthesis and cell wall structure were mapped genetically. Several candidate genes colocated with wood property QTL; however, these relationships must be verified in future experiments.
一项旨在绘制影响火炬松木材性质性状的数量性状基因座（QTL）的长期系列实验已经完成。这些实验旨在在多个遗传背景、环境和生长季节中识别并随后验证QTL。验证QTL对于证实观察到的标记-性状关联的生物学基础、提供QTL效应大小的精确估计以及预测给定年龄或特定环境下的QTL表达是必要的。验证基于在不同群体之间以及每个群体的多个生长季节中对QTL的重复检测。QTL的时间稳定性适中，大约一半在多个季节中被检测到。不同群体共有的QTL较少，但这些结果对于标记辅助选择的有限应用而言仍然令人鼓舞。来自较大群体的QTL比在较小群体中检测到的QTL解释的表型变异更少，这强调了进行使用更大家系的实验的必要性。此外，对18个与木质素生物合成和细胞壁结构相关的候选基因进行了遗传定位。几个候选基因与木材性质QTL共定位；然而，这些关系必须在未来的实验中加以验证。
