胃肠道恶性肿瘤中CD105表达与生长因子的关系及其意义
Relationship between expression of CD105 and growth factors in malignant tumors of gastrointestinal tract and its significance.
作者信息
Yu Jian-Xian, Zhang Xiao-Tun, Liao Yong-Qiang, Zhang Qi-Yi, Chen Hua, Lin Mei, Kumar Shant
机构信息
Department of Pathology, Qingdao Municipal Hospital, Qingdao 266011, Shandong Province, China.
出版信息
World J Gastroenterol. 2003 Dec;9(12):2866-9. doi: 10.3748/wjg.v9.i12.2866.
AIM
Angiogenesis is an important step in the growth of solid malignant tumors. A number of angiogenic factors have been found such as transforming growth factor beta1 (TGF-beta1) and vascular endothelial growth factor (VEGF). However, the roles of TGF-beta1 and VEGF in gastrointestinal carcinogenesis are still unclear. This study was to investigate the expressions of TGF-beta1 and VEGF in gastrointestinal tract malignant tumors, as well as their association with microvessel density (MVD). At the same time, we also observed the localization of TGF-beta1 and its receptor CD105 in gastric malignant tumors.
METHODS
The expressions of TGF-beta1 and CD105 were detected in 55 fresh specimens of gastric carcinoma and VEGF and CD105 in 44 fresh specimens of colorectal carcinoma by immunohistochemical staining (S-ABC). TGF-beta1 and CD105 in 55 gastric carcinoma tissues on the same slide were detected by using double-stain Immunohistochemistry (DS-ABC).
RESULTS
Among the 55 cases of gastric carcinoma tissues, 30 were positive for TGF-beta1 (54.55%). The MVD of TGF-beta1 strong positive group (++ approximately +++ 23.22 +/- 5.8) was significantly higher than that of weak positive group (+17.56 +/- 7.2) and negative group (- 17.46 +/- 3.9) (q=4.5, q=5.3207, respectively, P<0.01). In the areas of high expression of TGF-beta1, MVD and the expression of CD105 were also high. Among the 44 cases of colonic carcinoma tissues, 26 were positive for VEGF (59.1%). The expressions of both VEGF and CD105 (MVD) were related with the depth of invasion (F=5.438, P<0.05; F=4.168, P=0.05), lymph node metastasis (F=10.311, P<0.01; F=20.282, P<0.01) and Dukes stage (F=6.196, P<0.01; F=10.274, P<0.01), but not with histological grade (F=0.487, P>0.05). There was a significant correlation between the expression of VEGF and CD105 (MVD) (r=0.720, P<0.01).
CONCLUSION
Over-expression of TGF-beta1 and VEGF acts as stimulating factors of angiogenesis in gastrointestinal tumors. CD105, as a receptor of TGF-beta1, can regulate the biological effect of TGF-beta1 in tumor angiogenesis. MVD marked by CD105 is more suitable for detecting newborn blood vessels.
目的
血管生成是实体恶性肿瘤生长过程中的重要步骤。已发现多种血管生成因子，如转化生长因子β1（TGF-β1）和血管内皮生长因子（VEGF）。然而，TGF-β1和VEGF在胃肠道癌变中的作用仍不清楚。本研究旨在探讨TGF-β1和VEGF在胃肠道恶性肿瘤中的表达及其与微血管密度（MVD）的关系。同时，我们还观察了TGF-β1及其受体CD105在胃恶性肿瘤中的定位。
方法
采用免疫组织化学染色（S-ABC法）检测55例新鲜胃癌标本中TGF-β1和CD105的表达，以及44例新鲜结直肠癌标本中VEGF和CD105的表达。同一玻片上的55例胃癌组织采用双重免疫组织化学染色（DS-ABC法）检测TGF-β1和CD105。
结果
55例胃癌组织中，30例TGF-β1阳性（54.55%）。TGF-β1强阳性组（++～+++，23.22±5.8）的MVD显著高于弱阳性组（+，17.56±7.2）和阴性组（-，17.46±3.9）（q值分别为4.5、5.3207，P<0.01）。在TGF-β1高表达区域，MVD和CD105的表达也较高。44例结肠癌组织中，26例VEGF阳性（59.1%）。VEGF和CD105（MVD）的表达均与浸润深度（F=5.438，P<0.05；F=4.168，P=0.05）、淋巴结转移（F=10.311，P<0.01；F=,20.282，P<0.01）及Dukes分期（F=6.196，P<0.01；F=10.274，P<0.01）相关，但与组织学分级无关（F=0.487，P>0.05）。VEGF与CD105（MVD）的表达之间存在显著相关性（r=0.720，P<0.01）。
结论
TGF-β1和VEGF的过表达在胃肠道肿瘤血管生成中起刺激作用。CD105作为TGF-β1的受体，可调节TGF-β1在肿瘤血管生成中的生物学效应。以CD105标记的MVD更适合检测新生血管。
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