Griffin Alison, Callan Mary Beth, Shofer Frances S, Giger Urs
Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Am J Vet Res. 2003 Dec;64(12):1562-9. doi: 10.2460/ajvr.2003.64.1562.
To evaluate a canine D-dimer point-of-care (cD-d POC) test kit for use in healthy dogs and dogs with disseminated intravascular coagulation (DIC), thromboembolic disease (TED), and hemorrhage.
12 healthy dogs, 18 dogs with DIC, 23 dogs with TED (19 acute and 4 chronic), and 18 dogs with hemorrhage.
The cD-d POC, canine D-dimer ELISA (cD-d ELISA), human D-dimer latex agglutination (hD-d LA), and fibrin degradation product (FDP) tests were performed on citrated plasma.
All healthy dogs had negative cD-d POC test results and mean cD-d ELISA value of 0.2 U/mL. All dogs with DIC had positive cD-d POC test results and mean cD-d ELISA value of 44 U/mL. Dogs with acuteTED had a mean cD-d ELISA value of 34 U/mL, and 17 of 19 had positive cD-d POC test results. Mean cD-d ELISA value in dogs with hemorrhage was 14 units/mL, and 15 of 18 had positive cD-d POC test results. The cD-d ELISA values in dogs with hemorrhage were significantly higher than those of healthy dogs but lower than those of dogs with DIC and acute TED. The cD-d POC, cD-d ELISA, and hD-d LA tests were comparable in differentiating healthy dogs from dogs with DIC, acute TED, or hemorrhage and appeared to be superior to measurement of FDPs.
The cD-d POC test kit can be quickly and easily used and reliably detects dogs with DIC or acute TED. Positive results may also be seen in dogs with internal hemorrhage.
评估一种犬D - 二聚体即时检测（cD - d POC）试剂盒在健康犬以及患有弥散性血管内凝血（DIC）、血栓栓塞性疾病（TED）和出血的犬中的应用情况。
12只健康犬、18只患有DIC的犬、23只患有TED的犬（19只为急性，4只为慢性）以及18只患有出血的犬。
对枸橼酸盐血浆进行cD - d POC检测、犬D - 二聚体酶联免疫吸附测定（cD - d ELISA）、人D - 二聚体乳胶凝集试验（hD - d LA）以及纤维蛋白降解产物（FDP）检测。
所有健康犬的cD - d POC检测结果均为阴性，cD - d ELISA的平均数值为0.2 U/mL。所有患有DIC的犬的cD - d POC检测结果均为阳性，cD - d ELISA的平均数值为44 U/mL。患有急性TED的犬的cD - d ELISA平均数值为34 U/mL，19只中有17只cD - d POC检测结果为阳性。患有出血的犬的cD - d ELISA平均数值为14 U/mL，18只中有15只cD - d POC检测结果为阳性。患有出血的犬的cD - d ELISA数值显著高于健康犬，但低于患有DIC和急性TED的犬。cD - d POC、cD - d ELISA和hD - d LA检测在区分健康犬与患有DIC、急性TED或出血的犬方面具有可比性，并且似乎优于FDP的检测。
cD - d POC试剂盒使用快速简便，能够可靠地检测出患有DIC或急性TED的犬。患有内出血的犬也可能出现阳性结果。
