Shigenari Atsuko, Ando Asako, Renard Christine, Chardon Patrick, Shiina Takashi, Kulski Jerzy K, Yasue Hiroshi, Inoko Hidetoshi
Department of Molecular Life Science, Tokai University School of Medicine, Bohseidai, Isehara, 259-1193, Kanagawa, Japan.
Immunogenetics. 2004 Jan;55(10):695-705. doi: 10.1007/s00251-003-0627-0. Epub 2003 Dec 12.
Genome analysis of the swine leukocyte antigen ( SLA) region is needed to obtain information on the MHC genomic sequence similarities and differences between the swine and human, given the possible use of swine organs for xenotransplantation. Here, the genomic sequences of a 433-kb segment located between the non-classical and classical SLA class I gene clusters were determined and analyzed for gene organization and contents of repetitive sequences. The genomic organization and diversity of this swine non-class I gene region was compared with the orthologous region of the human leukocyte antigen ( HLA) complex. The length of the fully sequenced SLA genomic segment was 433 kb compared with 595 kb in the corresponding HLA class I region. This 162-kb difference in size between the swine and human genomic segments can be explained by indel activity, and the greater variety and density of repetitive sequences within the human MHC. Twenty-one swine genes with strong sequence similarity to the corresponding human genes were identified, with the gene order from the centromere to telomere of HCR - SPR1 - SEEK1 - CDSN - STG - DPCR1 - KIAA1885 - TFIIH - DDR - IER3 - FLOT1 - TUBB - KIAA0170 - NRM - KIAA1949 - DDX16 - FLJ13158 - MRPS18B - FB19 - ABCFI - CAT56. The human SEEK1 and DPCR1 genes are pseudogenes in swine. We conclude that the swine non-class I gene region that we have sequenced is highly conserved and therefore homologous to the corresponding region located between the HLA-C and HLA-E genes in the human.
鉴于猪器官可能用于异种移植，需要对猪白细胞抗原（SLA）区域进行基因组分析，以获取有关猪和人类主要组织相容性复合体（MHC）基因组序列异同的信息。在此，我们测定并分析了位于非经典和经典SLA I类基因簇之间的一个433 kb片段的基因组序列，以了解基因组织和重复序列的含量。将该猪非I类基因区域的基因组组织和多样性与人类白细胞抗原（HLA）复合体的直系同源区域进行了比较。已完全测序的SLA基因组片段长度为433 kb，而相应的HLA I类区域为595 kb。猪和人类基因组片段之间162 kb的大小差异可以通过插入缺失活性以及人类MHC中重复序列的更多种类和密度来解释。鉴定出了21个与相应人类基因具有高度序列相似性的猪基因，其从着丝粒到端粒的基因顺序为HCR - SPR1 - SEEK1 - CDSN - STG - DPCR1 - KIAA1885 - TFIIH - DDR - IER3 - FLOT1 - TUBB - KIAA0170 - NRM - KIAA1949 - DDX16 - FLJ13158 - MRPS18B - FB19 - ABCFI - CAT56。人类的SEEK1和DPCR1基因在猪中是假基因。我们得出结论，我们测序的猪非I类基因区域高度保守，因此与人类中位于HLA - C和HLA - E基因之间的相应区域同源。
