Kimura Masatomo, Furuta Tomoko, Hashimoto Shigeo, Takano Tetsuhiro, Nagao Koichi
Second Department of Pathology, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama 589-8511, Japan.
Acta Cytol. 2003 Nov-Dec;47(6):1099-102. doi: 10.1159/000326656.
Oncocytic carcinoma is a rare malignant tumor of the salivary gland. Abundant, granular, eosinophilic cytoplasm is recognized as an oncocytic feature that reflects an accumulation of mitochondria. Ultrastructural study or immunohistochemical staining using antimitochondrial antibody can confirm the oncocytic nature of the tumor. However, there have been no data on whether immunocytochemical staining for human mitochondria aids in the confirmation of the oncocytic nature of oncocytic carcinoma.
A 61-year-old man presented with a swelling in the left lower cheek. Computed tomography demonstrated a solid, isodense tumor in the parotid gland. An excisional biopsy of the tumor was performed, and an enlarged regional lymph node was removed. Imprint cytology of the lymph node showed cohesive cell clusters with lymphocytes. The clusters were composed of tumor cells that had characteristic abundant, granular cytoplasm and round to oval, centrally or eccentrically located nuclei with increased, fine chromatin and distinct nucleoli. Immunocytochemical staining revealed granular immunoreactivity of the cytoplasm for human mitochondria. Histology demonstrated tumor invasion in the normal gland and adjacent skeletal muscles. All tumor cells showed positive cytoplasm with antimitochondrial antibody by immunohistochemistry. Ultrastructural studies demonstrated packed mitochondria in the cytoplasm of the tumor.
Immunocytochemical staining for human mitochondria help confirm the oncocytic nature of oncocytic carcinoma in cytologic specimens.
嗜酸细胞癌是一种罕见的涎腺恶性肿瘤。丰富的颗粒状嗜酸性细胞质被认为是一种嗜酸细胞特征，反映了线粒体的聚集。超微结构研究或使用抗线粒体抗体的免疫组织化学染色可证实肿瘤的嗜酸细胞性质。然而，关于人线粒体免疫细胞化学染色是否有助于确认嗜酸细胞癌的嗜酸细胞性质，目前尚无相关数据。
一名61岁男性患者，左脸颊下部肿胀。计算机断层扫描显示腮腺内有一个实性等密度肿瘤。对肿瘤进行了切除活检，并切除了一个肿大的区域淋巴结。淋巴结印片细胞学检查显示有与淋巴细胞相连的细胞团。这些细胞团由肿瘤细胞组成，肿瘤细胞具有特征性的丰富颗粒状细胞质，细胞核呈圆形至椭圆形，位于中央或偏心位置，染色质增多、细腻，核仁明显。免疫细胞化学染色显示细胞质对人线粒体呈颗粒状免疫反应。组织学检查显示肿瘤侵犯正常腺体和邻近的骨骼肌。免疫组织化学检查显示所有肿瘤细胞的细胞质对抗线粒体抗体呈阳性。超微结构研究显示肿瘤细胞质中线粒体密集。
人线粒体免疫细胞化学染色有助于在细胞学标本中确认嗜酸细胞癌的嗜酸细胞性质。
