Tokutake Nobuya, Jing Bingwen, Cao Honghua, Regen Steven L
Department of Chemistry, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
J Am Chem Soc. 2003 Dec 24;125(51):15764-6. doi: 10.1021/ja038106s.
Nearest-neighbor recognition measurements have established that the effects of deuterium substitution on phospholipid mixing are exceedingly small. Thus, the mixing behavior of an exchangeable phospholipid bearing two stearoyl chains with a homologue containing two myristoyl chains in gel-fluid bilayers, fluid bilayers, cholesterol-rich fluid bilayers, and gel-fluid bilayers that have been enriched with cholesterol correspond to a difference in the free energy of mixing that is less than 2.2 cal/mol of hydrogen in all cases. These findings provide the strongest evidence to date in support of the use of deuterated phospholipids as "nonperturbing" probes for structural and dynamic studies of bilayer membranes.
最近邻识别测量结果表明，氘取代对磷脂混合的影响极其微小。因此，在凝胶-流体双层膜、流体双层膜、富含胆固醇的流体双层膜以及已富集胆固醇的凝胶-流体双层膜中，带有两条硬脂酰链的可交换磷脂与含有两条肉豆蔻酰链的同系物的混合行为，在所有情况下对应的混合自由能差异均小于每摩尔氢2.2卡。这些发现为目前使用氘代磷脂作为双层膜结构和动力学研究的“无扰动”探针提供了最有力的证据。
