磷脂的跨膜互补性。超越流动镶嵌模型的审视。

Transbilayer complementarity of phospholipids. A look beyond the fluid mosaic model.

作者信息

Zhang Jianbing, Jing Bingwen, Tokutake Nobuya, Regen Steven L

机构信息

Department of Chemistry, Lehigh University, Bethlehem, PA 18015, USA.

出版信息

J Am Chem Soc. 2004 Sep 8;126(35):10856-7. doi: 10.1021/ja046892a.

DOI:10.1021/ja046892a

PMID:15339166

Abstract

Lipid-lipid interactions across a phospholipid bilayer were probed by measuring the nearest-neighbor preferences of exchangeable phospholipid monomers derived from 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) in the presence of nonexchangeable DMPE- or DSPE-based dimers. Each of these permanent dimers promoted homophospholipid association to the same extent, whereas the corresponding nonexchangeable monomers were without effect. These results support a model in which the longer phospholipids in one monolayer leaflet preferentially associate with shorter ones in the adjoining monolayer. Such transbilayer complementarity is likely to play an important role in stabilizing biological membranes and also in promoting a compositional interdependence of their two lipid leaflets.

摘要

通过测量在不可交换的基于二肉豆蔻酰磷脂酰乙醇胺（DMPE）或二硬脂酰磷脂酰乙醇胺（DSPE）的二聚体存在下，源自1,2-二肉豆蔻酰-sn-甘油-3-磷酸乙醇胺（DMPE）和1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺（DSPE）的可交换磷脂单体的近邻偏好，来探究跨磷脂双分子层的脂-脂相互作用。这些永久性二聚体中的每一种都在相同程度上促进了同磷脂的缔合，而相应的不可交换单体则没有作用。这些结果支持了一种模型，即一个单层小叶中较长的磷脂优先与相邻单层中较短的磷脂缔合。这种跨双分子层互补性可能在稳定生物膜以及促进其两个脂质小叶的组成相互依赖方面发挥重要作用。