Nagel M, Richter F, Haring-Bolívar P, Kurz H
Institute for Semiconductor Electronics, Aachen University, Sommerfeldstr. 24, 52074 Aachen, Germany.
Phys Med Biol. 2003 Nov 21;48(22):3625-36. doi: 10.1088/0031-9155/48/22/001.
We report on a novel resonant THz sensor for the label-free analysis of DNA molecules. The sensor allows the direct detection of DNA-probe molecules at functionalized electrodes via hybridization. Subsequent time resolved photoconductive sampling of the THz transmission identifies the binding state between probe and target DNA. Integrating neighbouring sensors on a chip, this technique can be extended to a parallel analysis of multiple DNA sequences. A clearly readable sensor response is obtained with less then 40 fmol of 20-mer single-stranded DNA molecules, indicating at least a sevenfold sensitivity increase compared to previous approaches.
我们报道了一种用于DNA分子无标记分析的新型共振太赫兹传感器。该传感器可通过杂交直接检测功能化电极上的DNA探针分子。随后对太赫兹传输进行时间分辨光电导采样，可确定探针与目标DNA之间的结合状态。将相邻传感器集成在芯片上，该技术可扩展到对多个DNA序列的并行分析。使用不到40飞摩尔的20聚体单链DNA分子即可获得清晰可读的传感器响应，这表明与之前的方法相比，灵敏度至少提高了7倍。
