运用聚合酶链反应和斑点杂交试验对匈牙利鹅样本中的鹅圆环病毒感染进行诊断。

Diagnosis of goose circovirus infection in Hungarian geese samples using polymerase chain reaction and dot blot hybridization tests.

作者信息

Ball N W, Smyth J A, Weston J H, Borghmans B J, Palya V, Glávits R, Ivanics E, Dán A, Todd D

机构信息

Veterinary Sciences Division, Department of Agriculture and Rural Development for Northern Ireland, Stoney Road, Stormont, Belfast, UK.

出版信息

Avian Pathol. 2004 Feb;33(1):51-8. doi: 10.1080/03079450310001610613.

DOI:10.1080/03079450310001610613

PMID:14681068

Abstract

A polymerase chain reaction (PCR) and dot blot hybridization (DBH) test have been developed for the diagnosis of infection by a novel circovirus of geese (GoCV). These tests were applied to samples of bursae of Fabricius from sick and dead birds from commercial goose farms in Hungary. In this second report of the occurrence of circovirus infection in diseased geese, 103 of 214 (48.1%) and 37 of 150 (24.6%) birds, and 49 of 76 (64.5%) and 18 of 76 (23.7%) flocks were positive by PCR and DBH respectively. The sensitivity of the PCR test was such that 0.10 fg of virus DNA was detectable. The DBH test was less sensitive, only detecting larger amounts (40 pg) of DNA, but was used as a semi-quantitative method for detecting the presence of virus. The incidence of infection was affected by factors such as the age of the birds and rearing methods.

摘要

已开发出一种聚合酶链反应（PCR）和斑点印迹杂交（DBH）检测方法，用于诊断由一种新型鹅圆环病毒（GoCV）引起的感染。这些检测方法应用于匈牙利商业鹅场病死鹅的法氏囊样本。在这份关于病鹅中圆环病毒感染情况的第二篇报告中，通过PCR和DBH检测，分别有214只鸟中的103只（48.1%）和150只鸟中的37只（24.6%），以及76个鸡群中的49个（64.5%）和76个鸡群中的18个（23.7%）呈阳性。PCR检测的灵敏度可达可检测到0.10 fg的病毒DNA。DBH检测的灵敏度较低，只能检测到较大量（40 pg）的DNA，但用作检测病毒存在的半定量方法。感染发生率受鸟类年龄和饲养方式等因素影响。