Chen Chiou-Lin, Wang Peng-Xuan, Lee Min-Shiuh, Shien Jui-Hung, Shien Happy K, Ou San-Jai, Chen Chien-Hung, Chang Poa-Chun
Department of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan.
Avian Dis. 2006 Mar;50(1):92-5. doi: 10.1637/7435-090705R1.1.
This article reports the complete nucleotide sequences of four duck circovirus (DuCV) isolates from sick ducks in Taiwan and development of a polymerase chain reaction (PCR) for detection and differentiation of goose circovirus (GoCV) and DuCV. Sequence comparison showed that Taiwanese DuCV isolates had 82.5%-83.8% nucleotide sequence identity to the German and North American DuCV isolates. This is the first report on the presence of DuCV and its associated diseases outside Germany. A PCR test was developed using a universal primer pair based on conserved sequences present in the genomes of GoCV and DuCV. This PCR test could detect and differentiate between GoCV and DuCV by the size of PCR product each virus produced (256 bp for GoCV and 228 bp for DuCV). Application of this PCR test to samples of bursa of Fabricius from sick birds in the field showed that 9 of 26 goose samples contained GoCV, while 13 of 34 duck samples contained DuCV. This PCR test could serve as a fast and sensitive method for detection and differentiation of DuCV and GoCV.
本文报道了从台湾病鸭中分离出的4株鸭圆环病毒（DuCV）的完整核苷酸序列，并开发了一种用于检测和区分鹅圆环病毒（GoCV）与DuCV的聚合酶链反应（PCR）。序列比较表明，台湾DuCV分离株与德国和北美DuCV分离株的核苷酸序列同一性为82.5%-83.8%。这是德国以外首次关于DuCV及其相关疾病存在的报道。基于GoCV和DuCV基因组中存在的保守序列，使用一对通用引物开发了一种PCR检测方法。该PCR检测方法可通过每种病毒产生的PCR产物大小（GoCV为256 bp，DuCV为228 bp）来检测和区分GoCV与DuCV。将该PCR检测方法应用于田间病禽的法氏囊样本，结果显示，26份鹅样本中有9份含有GoCV，34份鸭样本中有13份含有DuCV。该PCR检测方法可作为一种快速、灵敏的检测和区分DuCV与GoCV的方法。
