Chekhun V F, Tryndiak V P, Todor I M, Mykhaĭlenko V M, Kondrychyn I H, Dovbeshko H I, Repnyts'ka O P, Kulyk H I
Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Science of Ukraine, Kyiv.
Ukr Biokhim Zh (1999). 2003 Jul-Aug;75(4):120-5.
The investigation is aimed to study qualitative and quantitative composition of phospholipids, cholesterol content and lipids unsaturation index in plasma membranes of Guerin's carcinoma cells sensitive or resistant to doxorubicin. The comparison of infrared spectra and phospholipids unsaturation index showed that the unsaturation level of fatty acids in plasma membrane from resistant cells was lower than that from sensitive carcinoma cells. 31P-NMR spectroscopy of plasma membranes phospholipids shows the increase of phosphatidylserine and sphingomyeline content in plasma membrane isolated from resistant tumor as compared with sensitive tumor. The levels of phosphatidylcholine and phosphatidylethanolamine were equal in drug-resistant and drug-sensitive carcinoma strains. Changes in plasma membrane from resistant cells result in elevation of plasma membrane microviscosity and phosphatidylserine level increase can suggest the activation of P-glycoprotein-mediated efflux of doxorubicin.
该研究旨在探讨对阿霉素敏感或耐药的格林氏癌细胞质膜中磷脂的定性和定量组成、胆固醇含量及脂质不饱和度指数。红外光谱和磷脂不饱和度指数的比较表明，耐药细胞质膜中脂肪酸的不饱和度水平低于敏感癌细胞。质膜磷脂的31P-NMR光谱显示，与敏感肿瘤相比，从耐药肿瘤中分离出的质膜中磷脂酰丝氨酸和鞘磷脂含量增加。耐药和药敏癌细胞株中磷脂酰胆碱和磷脂酰乙醇胺的水平相等。耐药细胞质膜的变化导致质膜微粘度升高，磷脂酰丝氨酸水平升高可能提示P-糖蛋白介导的阿霉素外排被激活。
