He C-L, Du J-L, Wu G-C, Lee Y-H, Sun L-T, Chang C-F
Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
Cytogenet Genome Res. 2003;101(3-4):309-13. doi: 10.1159/000074354.
Black porgy, Acanthopagrus schlegeli Bleeker, is a marine protandrous hermaphrodite fish. A Dmrt1 cDNA was cloned and characterized and in order to study the process of sex change in this species, mRNA transcripts of Dmrt1 were monitored. Dmrt1 was specifically transcribed in testis and seminal vesicle in 2-year-old black porgy according to RT-PCR and Southern analysis. A real-time quantification PCR analysis was further developed for the measurement of Dmrt1 transcripts. Dmrt1 transcripts were at significantly higher levels in bisexual testis than bisexual ovary in 1+ and 2+ year-old fish. Dmrt1 transcripts decreased in the functional and bisexual testis of 3-year-old fish. Much higher levels of Dmrt1 transcripts in the bisexual ovary were detected in 1+ year-old fish than in 2+ and 3-year-old fish. No differences in Dmrt1 transcripts were found in bisexual ovaries of 2+ and 3-year-old fish and female ovaries of 3-year-old fish. The data suggest there is relationship of Dmrt1 to the sex change of protandrous black porgy.
黑鲷（Acanthopagrus schlegeli Bleeker）是一种海洋雄性先熟的雌雄同体鱼类。克隆并鉴定了一个Dmrt1 cDNA，为研究该物种的性别转变过程，对Dmrt1的mRNA转录本进行了监测。根据逆转录聚合酶链反应（RT-PCR）和Southern分析，Dmrt1在2岁黑鲷的睾丸和精囊中特异性转录。进一步开发了实时定量PCR分析方法来测量Dmrt1转录本。在1岁和2岁的鱼中，双性睾丸中的Dmrt1转录本水平显著高于双性卵巢。在3岁鱼的功能性和双性睾丸中，Dmrt1转录本减少。在1岁鱼的双性卵巢中检测到的Dmrt1转录本水平远高于2岁和3岁鱼。在2岁和3岁鱼的双性卵巢以及3岁鱼的雌性卵巢中，未发现Dmrt1转录本有差异。数据表明Dmrt1与雄性先熟黑鲷的性别转变有关。
