Ener B, Douglas L J
Department of Microbiology, University of Glasgow, UK.
FEMS Microbiol Lett. 1992 Nov 15;78(1):37-42. doi: 10.1016/0378-1097(92)90284-u.
Adhesion of four isolates of Candida albicans to buccal epithelial cells was determined after growth of the yeasts in defined medium containing 50 mM glucose or 500 mM galactose as the carbon source. With each isolate, adhesion of galactose-grown yeasts was significantly higher than that of glucose-grown organisms. Yeast cell-surface hydrophobicity was assessed by two methods, a modified hydrocarbon adhesion assay and a more sensitive polystyrene microsphere assay. All four isolates were significantly more hydrophobic after growth on 500 mM galactose than after growth on 50 mM glucose. Overall, a strong positive correlation between adhesion and surface hydrophobicity was observed (r = 0.965). These results are discussed in relation to the role of yeast-surface hydrophobicity in pathogenesis.
在以50 mM葡萄糖或500 mM半乳糖作为碳源的限定培养基中培养酵母后，测定了4株白色念珠菌分离株对颊上皮细胞的黏附情况。对于每一株分离株，在半乳糖培养基中生长的酵母的黏附能力显著高于在葡萄糖培养基中生长的酵母。通过两种方法评估酵母细胞表面疏水性，一种是改良的碳氢化合物黏附试验，另一种是更灵敏的聚苯乙烯微球试验。所有4株分离株在500 mM半乳糖培养基中生长后比在50 mM葡萄糖培养基中生长后疏水性显著增强。总体而言，观察到黏附与表面疏水性之间存在很强的正相关性（r = 0.965）。结合酵母表面疏水性在发病机制中的作用对这些结果进行了讨论。
