Kennedy M J, Sandin R L
Microbiology and Nutrition Research Unit, Upjohn Company, Kalamazoo, Michigan 49001.
J Med Vet Mycol. 1988 Apr;26(2):79-92.
The effect of cultivation in 13 media (10 complex, and three synthetic), as well as altering growth conditions, on Candida albicans adhesion, cell surface hydrophobicity and cell wall ultrastructure was studied. Adhesion of C. albicans to buccal epithelial cells (BECs) was significantly modified by all of the factors tested, particularly growth medium. In general, optimal adhesive activity for C. albicans was observed when the cells were grown in defined media (depending on the carbohydrate used) and/or at 25 degrees C. Moreover, significant differences in adhesion to BECs were noted when C. albicans was grown in the same complex medium from different manufacturers and in different batches of medium from the same manufacturer. Electron microscopy revealed significant differences in surface topography and cell wall ultrastructure of C. albicans grown in different media but none of these differences, including presence or absence of an outer floccular layer, appeared to correlate with the adhesive changes noted, which raises questions regarding the location and nature of the Candida adhesin(s). Likewise, cell surface hydrophobicity could not be correlated with adhesion to BECs but may have influenced yeast coadhesion. The results indicate that Candida adhesion is highly dependent upon the cultivation conditions of the yeast cells tested, and may explain discrepancies in the literature regarding the biochemical nature of the surface component(s) responsible for C. albicans adhesion.
研究了13种培养基（10种复合培养基和3种合成培养基）中的培养以及改变生长条件对白色念珠菌黏附、细胞表面疏水性和细胞壁超微结构的影响。所有测试因素，尤其是生长培养基，均显著改变了白色念珠菌对颊上皮细胞（BECs）的黏附。一般来说，当细胞在特定培养基中生长（取决于所使用的碳水化合物）和/或在25摄氏度下生长时，观察到白色念珠菌具有最佳黏附活性。此外，当白色念珠菌在不同制造商生产的相同复合培养基中以及同一制造商不同批次的培养基中生长时，其对BECs的黏附存在显著差异。电子显微镜显示，在不同培养基中生长的白色念珠菌的表面形貌和细胞壁超微结构存在显著差异，但这些差异，包括是否存在外层絮状层，似乎均与所观察到的黏附变化无关，这引发了关于念珠菌黏附素的位置和性质的问题。同样，细胞表面疏水性与对BECs的黏附无关，但可能影响酵母共黏附。结果表明，念珠菌的黏附高度依赖于所测试酵母细胞的培养条件，这可能解释了文献中关于负责白色念珠菌黏附的表面成分生化性质的差异。
