Begum Ghousia
Fisheries Lab, Department of Zoology, College of Science, Osmania University, Hyderabad, AP, India.
Aquat Toxicol. 2004 Jan 7;66(1):83-92. doi: 10.1016/j.aquatox.2003.08.002.
The effect of carbofuran insecticide on certain metabolites and enzymes of protein and carbohydrate metabolism was evaluated in liver and muscle tissues of the freshwater fish, Clarias batrachus during exposure and following cessation of exposure. Thirty-six fish were exposed to 7.66mg of carbofuran/l for 6 days. After 6 days, 18 fish were transferred into carbofuran-free water for 6 days (recovery period) in order to study the recovery response. Eighteen fish were kept in clean water as controls. Liver and muscle tissues were isolated from exposed, recovery and control fish at the end of 1, 3 and 6 days in each condition, and used for the estimation of protein, amino acids, ammonia, glycogen, aminotransaminases, glycogen phosphorylase (a and ab) and glutamate dehydrogenase. Total protein showed a delayed decrease in liver and muscle tissues but recovered by the end of the recovery period. Free amino acid content was affected little in liver, but was elevated in muscle, and ammonia levels were elevated in both tissues throughout the exposure period, and ammonia levels in liver remained elevated during recovery. Glycogen content of liver declined substantially, and rebounded after transfer of fish into clean water. The activity levels of alanine aminotransaminase, aspartate aminotransaminase, glutamate dehydrogenase and glycogen phosphorylase a were found to increase in both tissues during the exposure period. The glycogen phosphorylase ab activity in liver was suppressed on exposure to carbofuran. The enzymes exhibited different recovery pattern in liver and muscle tissues of C. batrachus. The patterns of effect of carbofuran on intermediary metabolism suggest avenues to determine the mechanisms by which such effects occur.
在暴露期间及停止暴露后,评估了呋喃丹杀虫剂对淡水鱼胡子鲶肝脏和肌肉组织中蛋白质和碳水化合物代谢的某些代谢产物及酶的影响。36条鱼暴露于7.66毫克/升的呋喃丹中6天。6天后,将18条鱼转移到不含呋喃丹的水中6天(恢复期),以研究恢复反应。18条鱼饲养在清洁水中作为对照。在每种情况下,于第1、3和6天结束时,从暴露组、恢复组和对照组鱼中分离出肝脏和肌肉组织,用于蛋白质、氨基酸、氨、糖原、氨基转氨酶、糖原磷酸化酶(a和ab)以及谷氨酸脱氢酶的测定。总蛋白在肝脏和肌肉组织中呈现延迟下降,但在恢复期结束时恢复。肝脏中游离氨基酸含量受影响较小,但肌肉中游离氨基酸含量升高,并且在整个暴露期两个组织中的氨水平均升高,在恢复期肝脏中的氨水平仍保持升高。肝脏中的糖原含量大幅下降,在鱼转移到清洁水中后反弹。在暴露期,丙氨酸转氨酶、天冬氨酸转氨酶、谷氨酸脱氢酶和糖原磷酸化酶a的活性水平在两个组织中均升高。暴露于呋喃丹时,肝脏中的糖原磷酸化酶ab活性受到抑制。这些酶在胡子鲶的肝脏和肌肉组织中表现出不同的恢复模式。呋喃丹对中间代谢的影响模式为确定此类影响发生的机制提供了途径。