Mukhopadhyay P K, Mukherji A P, Dehadrai P V
Toxicology. 1982;23(4):337-45. doi: 10.1016/0300-483x(82)90071-3.
Biochemical changes in the air-breathing catfish, Clarias batrachus (Linn.) exposed to a sublethal level of carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate) at 0.5 ppm concentration in ambient water for a period of 30 days were assessed. A small reduction in growth rate was observed in the fish treated with 0.5 ppm carbofuran for 60 days although no mortality or any apparent symptom of toxicity could be noted. Studies were carried out on the activities of certain enzymes of intermediary metabolism viz., glucose 6-phosphatase, alkaline phosphatase, acid phosphatase, Na+, K+-ATPase, GOT and GPT in certain vital tissues of the fish exposed to carbofuran (0.5 ppm) for 30 days. Exposure to carbofuran resulted in sharp inhibition of acetylcholinesterase activity in brain of the fish which recovered rather rapidly after terminating pesticide treatment and maintaining the fish in clean freshwater. Ratio of the levels of calcium/phosphorus in serum showed significant diminution in experimental groups of fish compared to controls. Level of ammonia in serum of experimental fish was markedly increased while excretion of ammonia by fish showed concomitant decrease. The bioaccumulation level of the pesticide and its degraded product, 3-hydroxy-carbofuran in liver tissue was measured by gas chromatography. A rationale of the effect of carbofuran on metabolism vis-a-vis toxicity in the fish has been suggested.
评估了在环境水中以0.5 ppm浓度暴露于亚致死水平克百威（2,3 - 二氢 - 2,2 - 二甲基 - 7 - 苯并呋喃基甲基氨基甲酸酯）30天的呼吸空气的鲶鱼（Clarias batrachus (Linn.)）的生化变化。用0.5 ppm克百威处理60天的鱼生长速率略有下降，尽管未观察到死亡或任何明显的毒性症状。对暴露于克百威（0.5 ppm）30天的鱼的某些重要组织中的中间代谢的某些酶的活性进行了研究，即葡萄糖6 - 磷酸酶、碱性磷酸酶、酸性磷酸酶、Na + 、K + -ATP酶、谷草转氨酶和谷丙转氨酶。暴露于克百威导致鱼脑中乙酰胆碱酯酶活性急剧抑制，在终止农药处理并将鱼饲养在清洁淡水中后，该活性恢复得相当快。与对照组相比，实验组鱼血清中钙/磷水平的比率显著降低。实验鱼血清中的氨水平显著升高，而鱼的氨排泄量则相应减少。通过气相色谱法测量了肝脏组织中农药及其降解产物3 - 羟基克百威的生物累积水平。提出了克百威对鱼的代谢相对于毒性影响的原理。
