Liu Qin, Yan Xiyun, Li Yang, Zhang Ying, Zhao Xingang, Shen Yi
Center of Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, PR China.
Lab Invest. 2004 Feb;84(2):221-8. doi: 10.1038/labinvest.3700033.
The purpose of this study was to determine whether the expression of melanoma cell adhesion molecule (MCAM/CD146), like most other endothelial adhesion molecules, is reduced in the pregnancy disorder pre-eclampsia, and whether it is associated with the invasiveness of trophoblast. We used immunohistochemical approaches and analyzed 42 placentas from control and pre-eclamptic patients using an anti-CD146 monoclonal antibody. Our data show that in normal placentas, CD146 staining was specifically detected in intermediate trophoblasts that are most invasive and migratory, but not detected in noninvasive cytotrophoblasts or syncytiotrophoblasts. However in pre-eclampsia, CD146 was no longer present in the intermediate trophoblasts, although it was detectable in the blood vessels. At the same time, we tested CD31/PECAM-1, another endothelial adhesion molecule, and found its negative staining for all kinds of trophoblasts in both normal and pre-eclamptic placentas. The different staining patterns of CD146 in the normal and pre-eclamptic placentas provide the first evidence that in pre-eclampsia, intermediate trophoblasts fail to express CD146, implicating that CD146 plays an important role in trophoblast invasion.
本研究的目的是确定黑素瘤细胞黏附分子（MCAM/CD146）的表达是否像大多数其他内皮黏附分子一样，在妊娠疾病子痫前期会降低，以及它是否与滋养层细胞的侵袭性有关。我们采用免疫组化方法，使用抗CD146单克隆抗体分析了42例对照患者和子痫前期患者的胎盘。我们的数据表明，在正常胎盘中，CD146染色特异性地出现在侵袭性和迁移性最强的中间滋养层细胞中，而在非侵袭性的细胞滋养层细胞或合体滋养层细胞中未检测到。然而，在子痫前期，尽管在血管中可检测到CD146，但中间滋养层细胞中不再存在CD146。同时，我们检测了另一种内皮黏附分子CD31/PECAM-1，发现其在正常和子痫前期胎盘的各类滋养层细胞中均呈阴性染色。正常胎盘和子痫前期胎盘CD146的不同染色模式首次证明，在子痫前期，中间滋养层细胞无法表达CD146，这意味着CD146在滋养层细胞侵袭中起重要作用。
