Xu Ruqiang, Ye Xinfu, Quinn Li Qingshun
Department of Botany, Miami University, Oxford, OH 45056, USA.
Gene. 2004 Jan 7;324:35-45. doi: 10.1016/j.gene.2003.09.025.
We have identified and genetically characterized an Arabidopsis thaliana gene encoding a homolog of the Cleavage and Polyadenylation Specificity Factor (CPSF). This gene, named AtCPSF73-II, has been found to have a critical role in development by loss-of-function analysis using a Dissociation (Ds) insertion line SGT1922. The homozygous SGT1922 plants were lethal, but the heterozygous plants, while retaining their normal vegetative growth, displayed empty seed spaces as well as aborted seeds with embryos arrested at the globular stage. Genetic analysis indicated that the disruption of the AtCPSF73-II gene in SGT1922 plants caused severe reduction in genetic transmission of female gametes due to a loss of fertility, while the transmission of male gametes was normal. Two independent heterozygous lines with T-DNA insertion on the AtCPSF73-II gene also showed the similar phenotype. Gene expression analysis demonstrated that AtCPSF73-II was preferentially expressed in flowers. Protein sequence analysis revealed a group of AtCPSF73-II homologs with unknown function in animals, but not in yeast, which suggested a potential important function of this group of genes in the development of multicellular organisms.
我们已经鉴定并对拟南芥中一个编码切割与聚腺苷酸化特异性因子（CPSF）同源物的基因进行了遗传特征分析。这个名为AtCPSF73-II的基因，通过使用解离（Ds）插入系SGT1922进行功能缺失分析，已发现其在发育过程中起关键作用。纯合的SGT1922植株是致死的，但杂合植株在保持正常营养生长的同时，出现了空的种子空间以及胚胎停滞在球形期的败育种子。遗传分析表明，SGT1922植株中AtCPSF73-II基因的破坏由于育性丧失导致雌配子的遗传传递严重减少，而雄配子的传递正常。在AtCPSF73-II基因上有T-DNA插入的两个独立杂合系也表现出类似的表型。基因表达分析表明AtCPSF73-II在花中优先表达。蛋白质序列分析揭示了一组在动物中功能未知但在酵母中不存在的AtCPSF73-II同源物，这表明这组基因在多细胞生物发育中可能具有重要功能。
