Mithen F A, Ilyas A A, Birchem R, Cook S D
John Cochran VA Medical Center, St. Louis, MO.
J Neurol Sci. 1992 Oct;112(1-2):223-32. doi: 10.1016/0022-510x(92)90155-e.
Serum samples from 52 patients with the acute Guillain-Barré syndrome (GBS), 19 patients with other neurological disorders, and 18 healthy volunteers were tested for cytotoxicity in cultures of rat Schwann cells and dorsal root ganglion neurons. The samples were also examined by enzyme-linked immunosorbent assay for IgG and IgM antibodies against various acidic and neutral glycolipids. Samples from 16 of the 52 (31%) acute GBS patients and from 1 of the 6 patients with chronic inflammatory demyelinating polyneuropathy produced myelin breakdown in culture. Although 10 of the 16 cytotoxic acute GBS serum samples contained anti-glycolipid immunoglobulins, there was no correlation in individual samples between cytotoxic activity and the presence of antibodies against specific glycolipids. While our results do not exclude a role for anti-glycolipid antibodies in the pathogenesis of the acute GBS, the cytotoxic effects of acute GBS serum in cultures of Schwann cells and sensory neurons are probably not due to these antibodies alone.
对52例急性吉兰 - 巴雷综合征（GBS）患者、19例患有其他神经系统疾病的患者以及18名健康志愿者的血清样本，进行了大鼠雪旺细胞和背根神经节神经元培养中的细胞毒性检测。还通过酶联免疫吸附测定法检测了样本中针对各种酸性和中性糖脂的IgG和IgM抗体。52例急性GBS患者中的16例（31%）以及6例慢性炎症性脱髓鞘性多发性神经病患者中的1例的样本在培养中导致了髓鞘破坏。虽然16份具有细胞毒性的急性GBS血清样本中有10份含有抗糖脂免疫球蛋白，但单个样本中细胞毒性活性与针对特定糖脂的抗体存在情况之间并无相关性。虽然我们的结果不排除抗糖脂抗体在急性GBS发病机制中的作用，但急性GBS血清在雪旺细胞和感觉神经元培养中的细胞毒性作用可能并非仅由这些抗体所致。
