Lisak R P, Kuchmy D, Armati-Gulson P J, Brown M J, Sumner A J
Neurology. 1984 Sep;34(9):1240-3. doi: 10.1212/wnl.34.9.1240.
To study the anti-Schwann cell activity of serum from patients with Guillain-Barré syndrome (GBS), we used a 51Cr-release cytotoxicity assay. Specific release of 51Cr mediated by GBS serum did not differ from that with normals or disease controls. Heating sera from GBS patients or controls at 56 or 50 degrees C for 30 minutes markedly decreased 51Cr specific release, which could not be restored by addition of either 10% guinea pig serum or normal human serum. Thus, both GBS and control serum contain heat-labile serum factors that can induce cytotoxic reactions by Schwann cells.
为研究吉兰-巴雷综合征（GBS）患者血清的抗施万细胞活性，我们采用了51Cr释放细胞毒性试验。GBS血清介导的51Cr特异性释放与正常人和疾病对照者无差异。将GBS患者或对照者的血清在56或50摄氏度加热30分钟，可显著降低51Cr特异性释放，添加10%豚鼠血清或正常人血清均无法恢复。因此，GBS血清和对照血清均含有热不稳定血清因子，可诱导施万细胞产生细胞毒性反应。
