Leandro Nadja Suzana Mogyca, Gonzales Elisabeth, Ferro Jesus Aparecido, Ferro Maria Inês Tiraboschi, Givisiez Patrícia Emília Naves, Macari Marcos
Faculdade de Veterinária e Zootecnia, UNESP, São Paulo, Brazil.
Mol Reprod Dev. 2004 Feb;67(2):172-7. doi: 10.1002/mrd.10397.
This study evaluated the expression of heat shock protein 70 kD (hsp70) in broiler chicken embryos subjected to cold (Experiment I) or high incubation temperature (Experiment II). In each experiment, fertile eggs were distributed in three incubators kept at 37.8 degrees C. At day 13 (D13), D16, and D19 of incubation, the embryos were subjected to acute cold (32 degrees C) or heat (40 degrees C) for 4-6 hr. Immediately after cold or heat exposure, samples from the liver, heart, breast muscle, brain, and lungs of 40 embryos were taken per age and treatment (control or stressed embryos). A tissue pool from 10 embryos was used as 1 replication. The levels of hsp70 in each tissue sample was quantified by Western blot analysis. The data were analyzed in a 3 x 2 factorial arrangement of treatments with four replications. hsp70 was detected in all embryo tissues, and the brain contained 2- to 5-times more hsp70 protein compared to the other tissues in either cold or heat stressed embryos. hsp70 increases were observed in the heart and breast muscle of cold stressed embryos at D16 and D19, respectively. Heat stressed embryos showed an increase of hsp70 in the heart at D13 and D19, and in the lung at D19 of incubation. Younger embryos had higher hsp70 synthesis than older embryos, irrespective of the type of thermal stressor. The results indicate that the expression of hsp70 in broiler chicken embryos is affected by cold and heat distress, and is tissue- and age-dependent.
本研究评估了热休克蛋白70kD（hsp70）在遭受寒冷（实验I）或高温孵化（实验II）的肉鸡胚胎中的表达。在每个实验中，将受精蛋分布在三个保持在37.8摄氏度的孵化器中。在孵化的第13天（D13）、第16天和第19天，胚胎分别遭受急性寒冷（32摄氏度）或高温（40摄氏度）4 - 6小时。在寒冷或热暴露后立即从每个年龄和处理组（对照或应激胚胎）的40个胚胎的肝脏、心脏、胸肌、脑和肺中取样。来自10个胚胎的组织样本池用作1次重复。通过蛋白质印迹分析对每个组织样本中的hsp70水平进行定量。数据采用3×2析因处理设计，有4次重复。在所有胚胎组织中均检测到hsp70，在寒冷或热应激胚胎中，脑内的hsp70蛋白含量比其他组织多2至5倍。在寒冷应激胚胎中，分别在D16和D19时观察到心脏和胸肌中hsp70增加。热应激胚胎在孵化的D13和D19时心脏中hsp70增加，在D19时肺中hsp70增加。无论热应激源的类型如何，较年轻的胚胎比较年长的胚胎具有更高的hsp70合成。结果表明，肉鸡胚胎中hsp70的表达受热应激和冷应激影响，并且具有组织和年龄依赖性。
