[溴氰菊酯对大鼠脑内苄氧基试卤灵O-脱烷基酶活性及CYP2B1/2B2蛋白水平的影响]

[Effect of deltamethrin on benzyloxyresorufin O-dealkylatase activity and CYP2B1/2B2 protein level in rat brain].

作者信息

Liu Liegang, Yan Hong, Shi Nian, Liu Yugu, Yao Ping, Yang Xuefeng

机构信息

Department of Nutrition and Food Hygiene, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2002 Dec;20(6):422-4.

PMID:14694589

Abstract

OBJECTIVE

To detect the effect of deltamethrin (DM) on benzyloxyresorufin O-dealkylatase (BROD) activity and the expression of CYP2B1/2B2 in rat brain.

METHODS

BROD activity was determined by fluorophotometry under the treatment of DM in vivo and vitro. Western-blot analysis was used to detect the expression of CYP2B1/2B2.

RESULTS

In vivo, DM could markedly inhibit BROD activity in rat brain microsome after rats had been treated with DM (12.5 mg.kg-1.d-1, i.p.) for 5 days. The inhibitory rate in whole brain, cerebral cortex and cerebellum were 26.7%, 23.8% and 33.3% respectively(P < 0.05). However, in vitro, under the concentration of 2 x 10(-8)-2 x 10(-4) mol/L of DM, there was no obvious change of BROD activity in rat brain. Moreover, Western-blot analysis indicated that DM could significantly reduce the expression of CYP2B1/2B2 in vivo, the inhibitory rate of protein synthesis was 42.6%.

CONCLUSION

DM could inhibit BROD activity in rat brain and this effect may be related to the reduction of CYP2B1/2B2 protein synthesis.

摘要

目的

检测溴氰菊酯（DM）对大鼠脑内苄氧基试卤灵O - 脱烷基酶（BROD）活性及细胞色素P450 2B1/2B2（CYP2B1/2B2）表达的影响。

方法

采用荧光光度法测定体内、外DM处理后大鼠脑内BROD活性；采用蛋白质免疫印迹法检测CYP2B1/2B2的表达。

结果

体内实验中，大鼠腹腔注射DM（12.5 mg·kg-1·d-1）5天后，DM可显著抑制大鼠脑微粒体中BROD活性。全脑、大脑皮质及小脑的抑制率分别为26.7%、23.8%和33.3%（P < 0.05）。然而，体外实验中，在2×10(-8)-2×10(-4) mol/L浓度的DM作用下，大鼠脑内BROD活性无明显变化。此外，蛋白质免疫印迹分析表明，DM可显著降低体内CYP2B1/2B2的表达，蛋白质合成抑制率为42.6%。