Ji Zhi-ying, Shi Nian, Wang Su-qing, Dong Jie, Chen Mao-shan
Department of Environmental Toxicology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2003 Jun;21(3):197-9.
To study the effects of pyrethroids on the activity of gamma-aminobutyric acid transferase (GABAT) in rat brain.
The coupled enzyme ultraviolet spectrophotography was applied to observe the effects of deltamethrin (DM) and permethrin (PM) on the activities of GABAT in rat cerebral cortex, hippocampus, corpus striatum and cerebellum in vitro and in vivo.
In vitro, DM and PM had no significant effects on the activities of GABAT in rat cerebral cortex, hippocampus, corpus striatum and cerebellum at the final concentration of 10(-9) - 10(-4) mol/L. When 37.5 mg/kg DM and 600 mg/kg PM were orally administrated to the rats at one time, the activities of GABAT in rat cerebral cortex, hippocampus and cerebellum in the DM group [(2.96 +/- 0.43), (2.13 +/-0.44), (5.12 +/- 1.36) nmol x mg pro(-1) x min(-1), respectively] were lower than those in the control group [(3.43 +/- 0.41), (2.68 +/- 0.47), (6.74 +/- 1.64) nmol x mg pro(-1) x min(-1)] (P < 0.05), and the activities of GABAT in rat cerebral cortex and hippocampus in the PM group [(4.57 +/- 0.30), (4.18 +/- 0.63) nmol.mg pro(-1) x min(-1), respectively] were higher than those in the control group (P < 0.05). When 12.5 mg/kg DM and 200 mg/kg PM were orally administrated to the rats once a day for consecutive five days, the two pesticides had no significant effects on the activities of GABAT in rat cerebral cortex, hippocampus, corpus striatum and cerebellum (P > 0.05).
In vitro, DM and PM had no significant effects on the activity of GABAT in rat brain; in vivo, DM and PM may have different effects on the activity of GABAT in rat brain, which deserve further study.
研究拟除虫菊酯对大鼠脑内γ-氨基丁酸转移酶（GABAT）活性的影响。
采用偶联酶紫外分光光度法，观察溴氰菊酯（DM）和氯菊酯（PM）在体外和体内对大鼠大脑皮质、海马、纹状体及小脑GABAT活性的影响。
体外实验中，终浓度为10^(-9) - 10^(-4) mol/L时，DM和PM对大鼠大脑皮质、海马、纹状体及小脑的GABAT活性无显著影响。一次性给大鼠灌胃37.5 mg/kg DM和600 mg/kg PM后，DM组大鼠大脑皮质、海马及小脑的GABAT活性[分别为(2.96 ± 0.43)、(2.13 ± 0.44)、(5.12 ± 1.36) nmol·mg蛋白^(-1)·min^(-1)]低于对照组[分别为(3.43 ± 0.41)、(2.68 ± 0.47)、(6.74 ± 1.64) nmol·mg蛋白^(-1)·min^(-1)]（P < 0.05）；PM组大鼠大脑皮质和海马的GABAT活性[分别为(4.57 ± 0.30)、(4.18 ± 0.63) nmol·mg蛋白^(-1)·min^(-1)]高于对照组（P < 0.05）。连续5天每天给大鼠灌胃12.5 mg/kg DM和200 mg/kg PM后，两种农药对大鼠大脑皮质、海马、纹状体及小脑的GABAT活性均无显著影响（P > 0.05）。
体外实验中，DM和PM对大鼠脑内GABAT活性无显著影响；体内实验中，DM和PM对大鼠脑内GABAT活性可能有不同影响，值得进一步研究。
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