Zhong Tao, Chen Qing, Wu Ruiying, Yao Gengdong, Lu Deqiang, Chiang Huai
Bioelectromagnetic Lab., Zhejiang University School of Medicine, Hangzhou 310031, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2002 Aug;20(4):249-51.
To clone and identify MF-1 gene which responded to extremely low frequency magnetic fields(ELF MF) in Daudi cells, and explore the response universality of MF-1 gene in several MF-sensitive cell lines, so as to provide experimental basis for revealing the mechanism of biological effects induced by magnetic field.
The DNA fragment of MF-1 was cloned and sequenced; the mRNA level of MF-1 gene were analysed in MF-sensitive cell lines(HL-60, L1210 and CHL) by Northern blot after these cells being treated with 0.1 mT and 0.8 mT MF for 20 minutes and 24 hours, respectively.
The MF-1 cDNA sequence had 100% homology with cytochrome oxidase subunit 1 gene(CO1) by searching Gene Bank database; the transcription of CO1 in HL-60, L1210 and CHL cell lines which exposed to 0.1 mT and 0.8 mT MF for 20 minutes were significantly lower(0.38 +/- 0.12 and 0.37 +/- 0.04) than that of control(0.58 +/- 0.12) and so did for 24 hours exposure(0.46 +/- 0.09 and 0.45 +/- 0.09 vs 0.65 +/- 0.06) (P < 0.05).
CO1 is a MF-responsive gene. Cytochrome oxidase activity may be affected through low level of CO1 transcription by magnetic fields, thus induce bioeffects in organisms.
克隆并鉴定在Daudi细胞中对极低频磁场（ELF MF）产生响应的MF-1基因，探讨MF-1基因在几种对磁场敏感的细胞系中的响应普遍性，为揭示磁场诱导生物效应的机制提供实验依据。
克隆MF-1的DNA片段并测序；分别用0.1 mT和0.8 mT的MF处理MF敏感细胞系（HL-60、L1210和CHL）20分钟和24小时后，通过Northern印迹分析MF-1基因的mRNA水平。
通过搜索基因库数据库，MF-1 cDNA序列与细胞色素氧化酶亚基1基因（CO1）具有100%的同源性；暴露于0.1 mT和0.8 mT MF 20分钟的HL-60、L1210和CHL细胞系中CO1的转录水平（分别为0.38±0.12和0.37±0.04）显著低于对照组（0.58±0.12），暴露24小时的情况也是如此（分别为0.46±0.09和0.45±0.09对比0.65±0.06）（P<0.05）。
CO1是一个对磁场响应的基因。磁场可能通过低水平的CO1转录影响细胞色素氧化酶活性，从而在生物体中诱导生物效应。
